| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
We have developed a system to control the light-swichable gene expression by using photoreceptive molecules. In order to polish this system toward application as a novel biological tool, we further characterize CRY photoreception and interaction proteins in this study. We have detected the positive fluorescent signals on the plasma membrane of mammalian cultured cells. We found relatively high expression levels of chicken cryptochrome in a constitutive expression system using budding yeast. We also succeeded in the purification of the cryptochrome protein having the chromophore FAD, investigation of the spectral properties, and detection of in vitro structural changes induced by light.
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