Study on structure-function relationship of KcsA channel by single molecule manipulation.
| Project/Area Number |
24657110
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Okayama University (2013)
The Graduate School for the Creation of New Photonics Industries (2012) |
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Principal Investigator |
IDE Toru 岡山大学, 自然科学研究科, 教授 (60231148)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | イオンチャネル / １分子計測 / 単一チャネル電流 / 1分子計測 / 人工膜 / 蛍光 / 1分子操作 / kcsa |
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| Research Abstract |
Our purpose was to develop an apparatus for simulataneous mechanical manipulation and optical recording of single ion channel protein, KcsA, by which we measure inter- and intra-molecule interactions that activate the channel. We have succeeded in (1)single molecule manipulation of KcsA immobilized at the tip of a fine glass needle (2)developing a technique for mechanically incorporating the KcsA into an artificial bilayer (3)making the measurement efficiency much higher than conventional methods by optimizing experimental conditions.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Lab on a Chip2012
Author(s)
Y. Oyama
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Journal Title
A glass fiber sheet-based electroosmotic lateral flow immunoassay for point of care testing.
Volume: 12
Issue: 24
Pages: 5155-5159
DOI
Related Report
Peer Reviewed
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