Visualization of Coulomb potential by electron crystallography
| Project/Area Number |
24657111
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
YONEKURA Koji 独立行政法人理化学研究所, 放射光科学総合研究センター, 准主任研究員 (50346144)
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| Co-Investigator(Kenkyū-buntansha) |
MAKI Saori (YONEKURA Saori) 独立行政法人理化学研究所, 放射光科学総合研究センター, 研究員 (20513386)
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| Co-Investigator(Renkei-kenkyūsha) |
TOYOSHIMA Chikashi 東京大学, 分子細胞生物学研究所, 教授 (70172210)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 構造生物学 / 結晶構造解析 / 電子顕微鏡 / 膜蛋白質 / 超分子複合体 / Ca2+-ATPase / カタラーゼ |
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| Outline of Final Research Achievements |
Electron crystallography has the potential to analyze crystals of membrane proteins and macromolecular complexes too small or too thin for X-ray crystallography, as electrons are scattered 4 - 5 orders of magnitude more strongly than X-rays. Electron crystallography yields Coulomb potential maps, rather than electron density maps as X-rays do, providing information on charged states of amino-acids and metals. We presented such Coulomb potential maps at 3.4 and 3.2 Angstrom resolution, respectively, of calcium-ATPase and catalase obtained from crystals of just a few layers thick. These maps demonstrate that it is indeed possible to build atomic models from such crystals and charge information is included, often critical in understanding protein function.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Single shot three-dimensional imaging of high-index-faceted nanocrystals with femtosecond X-ray free electron laser pulses2014
Author(s)
R. Xu, H. Jiang, C. Song, Z. Huang, J. A. Rodriguez, C-C. Chen, D. Nam, J. Park, M. Gallagher-Jones, S. Kim, S. Kim, A. Suzuki, Y. Takayama, T. Oroguchi, Y. Takahashi, J. Fan, Y. Zou, T. Hatsui, Y. Inubushi, T. Kameshima, K. Yonekura et al.
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Journal Title
Nat. Commun.
Volume: 5
Issue: 1
Pages: 4061-4061
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] X線自由電子レーザーを用いた非結晶粒子のコヒーレントX 線回折イメージング2014
Author(s)
中迫雅由, 苙口友隆, 関口優希, 小林周, 橋本早紀, 白濱圭也, 山本雅貴, 高山裕貴, 米倉功治, 眞木さおり, 引間孝明, 高橋幸生, 鈴木明大, 松永幸大, 乾弥生, 登野健介, 亀島敬, 城地保昌, 犬伏雄一, 星貴彦
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Journal Title
日本結晶学会誌
Volume: 56
Pages: 27-35
NAID
Related Report
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[Journal Article] Post-transcriptional regulator Hfq binds catalase HPII: crystal structure of the complex2013
Author(s)
Yonekura, K., Watanabe, M., Kageyama, Y., Hirata, K., Yamamoto, M. and Maki-Yonekura, S.
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Journal Title
PLOS ONE (2013)
Volume: 8
Issue: 11
Pages: e78216-e78216
DOI
Related Report
Peer Reviewed
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[Journal Article] Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus2013
Author(s)
Staals, R. H. J., Agari, Y., Maki-Yonekura, S., Zhu, Y., Taylor, D. W., van Duijn, E., Barendregt, A., Vlot, M., Koehorst, J. J., Sakamoto, K., Masuda, A., Dohmae, N., Schaap, P. J., Doudna, J. A., Heck, A. J. R., Yonekura, K., van der Oost, J. and Shinkai, A.
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Journal Title
Molecular Cell
Volume: 52
Issue: 1
Pages: 135-145
DOI
Related Report
Peer Reviewed
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