| Project/Area Number |
24657114
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Hirosaki University |
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Principal Investigator |
MUTO Akira 弘前大学, 農学生命科学部, 研究員 (80034635)
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| Co-Investigator(Kenkyū-buntansha) |
CHISATO Ushida 弘前大学, 農学生命科学部, 准教授 (50250593)
姫野 俵太 弘前大学, 農学生命科学部, 教授 (80208785)
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| Co-Investigator(Renkei-kenkyūsha) |
HYOUTA Himeno 弘前大学, 農学生命科学部, 教授 (80208785)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | tRNA / mRNA / small RNA / E. coli / translation / transcription / 低分子RNA / 大腸菌 / protein / O157 / 遺伝子発現 / 翻訳 / 転写 / タンパク質 / ストレス |
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| Outline of Final Research Achievements |
We have found a tRNA-like sequence, which is highly conserved in the genomes of all the strains of Escherichia coli. Experiments to see the product of the sequence revealed that this is a part of a gene encoding a short protein. This is the first discovery of the bacteria gene including tRNA-like sequence.
We have prepared the mutant strain lacking the tRNA-like sequence, and the growth of the deletion mutant was compered with the wild-type strain under various culture conditions. The results showed that the deletion mutant cells are more sensitive to several antibiotics, such as streptomycin, kanamycine etc., which specifically interact with A-site of 30S ribosome, suggesting that the gene product is somehow involved in the translation process in the cell.
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