Function of a novel tRNA-like small RNA
| Project/Area Number |
24657114
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Hirosaki University |
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Principal Investigator |
MUTO Akira 弘前大学, 農学生命科学部, 研究員 (80034635)
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| Co-Investigator(Kenkyū-buntansha) |
CHISATO Ushida 弘前大学, 農学生命科学部, 准教授 (50250593)
姫野 俵太 弘前大学, 農学生命科学部, 教授 (80208785)
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| Co-Investigator(Renkei-kenkyūsha) |
HYOUTA Himeno 弘前大学, 農学生命科学部, 教授 (80208785)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | tRNA / mRNA / small RNA / E. coli / translation / transcription / 低分子RNA / 大腸菌 / protein / O157 / 遺伝子発現 / 翻訳 / 転写 / タンパク質 / ストレス |
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| Outline of Final Research Achievements |
We have found a tRNA-like sequence, which is highly conserved in the genomes of all the strains of Escherichia coli. Experiments to see the product of the sequence revealed that this is a part of a gene encoding a short protein. This is the first discovery of the bacteria gene including tRNA-like sequence.
We have prepared the mutant strain lacking the tRNA-like sequence, and the growth of the deletion mutant was compered with the wild-type strain under various culture conditions. The results showed that the deletion mutant cells are more sensitive to several antibiotics, such as streptomycin, kanamycine etc., which specifically interact with A-site of 30S ribosome, suggesting that the gene product is somehow involved in the translation process in the cell.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence.2014
Author(s)
Yang, Z., Guo, Q., Goto, S., Chen, Y., Li, N., Yan, K., Zhang, Y., Muto, A., Deng, H., Himeno, H., Lei, J. & Gao, N.:
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Journal Title
Protein Cell
Volume: 5
Pages: 394-407
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process.2013
Author(s)
Guo, Q., Goto, S., Chen, Y., Feng, B., Xu, Y., Muto, A., Himeno, H., Deng, H., Lei, J., Gao, N.
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Journal Title
Nucleic Acids Res.
Volume: 41
Pages: 2609-2620
DOI
Related Report
Peer Reviewed
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[Journal Article] Rice Annotation Project Database (RAP-DB): An integrative and interactive database for rice genomics.2013
Author(s)
Sakai H, Lee SS, Tanaka T, Numa H, Kim J, Kawahara Y, Wakimoto H, Yang CC, Iwamoto M, Abe T, Yamada Y, Muto A, Inokuchi H, Ikemura T, Matsumoto T, Sasaki T, Itoh T.
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Journal Title
Plant and Cell Physiology
Volume: 52
DOI
Related Report
Peer Reviewed
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