| Project/Area Number |
24657115
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SASAKI Hiroshi 東京大学, 分子細胞生物学研究所, 助教 (30611454)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | RNA / 1分子イメージング / RNAサイレンシング / 核酸 / RISC / Argonaute / 複合体形成 / 全反射顕微鏡 |
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| Outline of Final Research Achievements |
RNA silencing, such as RNA interference and gene silencing by microRNAs, is a gene regulation system universally conserved in eukaryotes. The RNA-induced silencing complex, which is composed of an Argonaute protein and a single-stranded RNA, plays a central role in this system. However, the detailed mechanisms of RISC assembly remained elusive. In this project, by single-molecule imaging approach using total-internal reflection microscopy, I have tried to visualize how RISC is assembled, and revealed the fundamental steps in RISC assembly.
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