| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
TFIID, the largest GTF composed of TBP and 14 TAFs, and its related complex SAGA play critical roles in transcriptional activation by regulating TBP-DNA interactions. Previously,we suggested an intriguing possibility that CLN2 mRNA generated by TFIID or SAGA (designated as CLN2 mRNA[TFIID] or [SAGA], respectively) could produce two types of Cln2p that carry distinct functions.
Here we demonstrate that TAND (Taf1p N-terminal domain) of TFIID may determine the production ratio between CLN2 mRNA[TFIID] and [SAGA], and also that the latter mRNA may be specifically regulated by Ssd1p and RAM (Regulation of Ace2p activity and cellular Morphogenesis) signaling pathway at the translational level. These results support the idea that the selection of core transcription factor (e.g. TFIID versus SAGA) may affect the fate of mRNA generated even from a single promoter.
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