| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
To elucidate functions of retrotransposon sequences inserted in the untranslated region of mRNAs, we performed a series of analyses on an abundant nuclear noncoding RNA called 4.5SH, which is highly homologous to retrotransposon SINE B1. 4.5SH formed double-stranded RNA duplex with mRNAs containing antisense insertions of SINE B1, which were retained in the nucleus. The nuclear-retained mRNAs with antisense SINE B1 insertions were released into cytoplasm upon knockdown of 4.5SH. The nuclear retention via 4.5SH was regulated by a double-stranded RNA binding protein called NF110. These results revealed a novel mechanism that controls sub-cellular localization of mRNAs through the intra-molecular formation of the double stranded RNA structures.
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