Elucidation of molecular mechanisms controlling localization and metabolism of mRNAs via retrotransposon insertions
Project/Area Number |
24657123
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
|
Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
NAKAGAWA Shinichi 独立行政法人理化学研究所, 中川RNA生物学研究室, 准主任研究員 (50324679)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | ノンコーディングRNA / レトロトランスポゾン / 4.5SH / 核 / SINE B2 / NF110 / CRISPR-Cas9 |
Outline of Final Research Achievements |
To elucidate functions of retrotransposon sequences inserted in the untranslated region of mRNAs, we performed a series of analyses on an abundant nuclear noncoding RNA called 4.5SH, which is highly homologous to retrotransposon SINE B1. 4.5SH formed double-stranded RNA duplex with mRNAs containing antisense insertions of SINE B1, which were retained in the nucleus. The nuclear-retained mRNAs with antisense SINE B1 insertions were released into cytoplasm upon knockdown of 4.5SH. The nuclear retention via 4.5SH was regulated by a double-stranded RNA binding protein called NF110. These results revealed a novel mechanism that controls sub-cellular localization of mRNAs through the intra-molecular formation of the double stranded RNA structures.
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Report
(4 results)
Research Products
(10 results)