Analysis of the possible contribution of perichromosomal field to the chromosomal stability

• Project/Area Number: 24657124
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: MASATOSHI Takagi 独立行政法人理化学研究所, 今本細胞核機能研究室, 専任研究員 (60324779)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 分裂期染色体 / 分裂期 / 脱リン酸化酵素 / 細胞分裂期 / 染色体

Research Abstract

The outer layer around each mitotic chromosome, which we call PCF for 'perichromosomal field', is a cellular region with unknown functions. Derived from the knowledge obtained from the functional analyses of Ki67 antigen, a major constituent of PCF, we showed the possibility that the PCF might be involved in the 'quality' of the progression of mitosis. The PCF seems to contribute to the efficient progression of mitosis by providing a 'field' for factors such as PP1 gamma; (protein phosphtase 1 gamma;) to localize on mitotic chromosomes in appropriate timing. The PCF might have function also in supporting the structure of mitotic chromosomes from their outside.

Research Products

• [Journal Article] Ki-67 is a PP1-interacting protein that organizes the mitotic chromosome periphery (2014)
  • Author(s): Booth, D., Takagi, M., Sanchez-Pulido, L., Petfalski, E., Vargiu, G., Samejima, K., Imamoto, N., Ponting, C., Tollervey, D., Earnshaw, W., and Vagnerelli, P.
  • Journal Title: eLife Volume: 27;3
  • DOI: 10.7554/elife.01641
  • Peer Reviewed
• [Journal Article] Control of Nuclear Size by NPC Proteins (2014)
  • Author(s): Takagi, M., and Imamoto, N.
  • Journal Title: Advances in Experimental Medicine and Biology Volume: Vol. 773 Pages: 571-591
• [Journal Article] Control of Nuclear Size by NPC Proteins (2014)
  • Author(s): Masatoshi Takagi, Naoko Imamoto
  • Journal Title: Advances in Experimental Medicine and Biology Volume: 773 Pages: 571-591
  • DOI: 10.1007/978-1-4899-8032-8_26
  • ISBN: 9781489980311, 9781489980328
• [Presentation] Ki67抗原により染色体表層領域へリクルートされるPP1γの役割 (2013)
  • Author(s): 高木昌俊
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2013-12-05
• [Presentation] Ki67抗原により染色体表層領域へリクルートされるPP1γの役割 (2013)
  • Author(s): 高木昌俊、田口温子、西山祐子、今本尚子
  • Organizer: 第６３回日本分子生物学会
  • Place of Presentation: 兵庫 神戸ポートアイランド
  • Invited
• [Presentation] Ki67抗原により染色体表層領域へリクルートされるPP1γの役割 (2012)
  • Author(s): 高木昌俊
  • Organizer: 第30回染色体ワークショップ
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2012-12-20
• [Presentation] Ki67抗原により染色体表層領域にリクルートされるPP1gの役割 (2012)
  • Author(s): 高木 昌俊
  • Organizer: 第３０回染色体ワークショップ・第１１回核ダイナミクス研究会（合同開催）
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県）
• [Remarks] 今本細胞核機能研究室ホームページ
  • URL: http://www.riken.jp/celldynamics/index.html
• [Remarks] 今本細胞核機能研究室ホームページ
  • URL: http://www.riken.jp/celldynamics/index.html