Study of resistance capacitation mechanism against actin polymerization inhibitors in Tetrahymena
| Project/Area Number |
24657128
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
NUMATA Osamu 筑波大学, 生命環境系, 教授 (50189354)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | テトラヒメナ / アクチン / ラトランキュリンA / 薬剤耐性 / 細胞質分裂 / 食胞形成 / 遺伝子過剰発現株 / ゾウリムシ / 遺伝子破壊株 / コフィリン |
|---|
| Outline of Final Research Achievements |
For the solution to the molecular mechanism of "specific drug resistance capacitation against actin polymerization inhibitors in Tetrahymena thermophila", we focused on the actin gene Act2. Its expression amount was increased by actin polymerization inhibitor Lat-A. We destroyed the Act2 gene. In Act2 gene disrupted strain, it could not acquire the drug-resistant ability. Thus, by expression of the Act2, it concludes that specific drug resistance ability against actin polymerization inhibitors is acquired. In addition, in nutrition growth phase, a treatment of Lat-A to Act2 gene KO strain inhibited progression from a G2 phase to a mitotic phase. In starvation, the treatment of Lat-A to Act2 gene KO strain inhibited autophagy. Moreover, because there was actin polymerization inhibitor resistance ability in Paramecium tetraurelia, it suggests that this nature is common in ciliates.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.2015
Author(s)
Funakoshi, S., Shimizu, T., Numata, O., Ato, M., Melchers, F., and Ohnishi, K.
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Journal Title
PLoS One
Volume: 10
Issue: 1
Pages: e0117566-e0117566
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] ADF/cofilin is not essential but is crucially important for actin activities during phagocytosis in Tetrahymena thermophila.2013
Author(s)
Shiozaki, N., Nakano, K., Kushida, Y., Noguchi, T.Q.P., Uyeda, T.Q.P., Wloga, D., Dave, D., Vasudevan, K. K., Gaertig, J., Numata, O.
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Journal Title
Eukaryotic Cell
Volume: 12
Issue: 8
Pages: 1080-1086
DOI
Related Report
Peer Reviewed
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[Journal Article] Black Tea High-Molecular-Weight Polyphenol Stimulates Exercise Training-Induced Improvement of Endurance Capacity in Mouse via the Link between AMPK and GLUT4.2013
Author(s)
Eguchi, T., Kumagai, C., Fujihara, T., Takemasa, T., Ozawa, T., and Numata, O.
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Journal Title
PLoS ONE
Volume: 8
Issue: 7
Pages: e69480-e69480
DOI
NAID
Related Report
Peer Reviewed
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