Elucidation of molecular mechanisms involved in the regulation of the amount of cellular lipids

• Project/Area Number: 24657131
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Kyushu University (2013-2014); Kyoto University (2012)
• Principal Investigator: IKENOUCHI Junichi 九州大学, 理学(系)研究科(研究院), 准教授 (10500051)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 細胞膜脂質 / 脂質量 / リン脂質 / 脂質代謝酵素 / FANTOM4 / 細胞膜 / THP-1細胞 / 生合成 / ダイアシルグリセロール / 生体膜 / THP-1

Outline of Final Research Achievements

The amount of cellular lipids need to be accurately controlled. The membranes of mammalian cells contain several thousands of different phospholipid species and their metabolic pathways are very complicated. In this research proposal, I aimed to clarify the rate-determining steps of these metabolic network of phospholipid species in the regulation of the amount of cellular lipids. I revealed that the biosynthesis of diacylglycerol is highly up-regulated when the total amount of cellular lipids is increased.

Research Products

• [Journal Article] EPLIN is a crucial regulator for extrusion of RasV12-transformed cells. (2015)
  • Author(s): Ohoka, A., Kajita, M., Ikenouchi, J., Yako, Y., Kitamoto, S., Kon, S., Ikegawa, M., Shimada, T., Ishikawa, S., and Fujita, Y.
  • Journal Title: Journal of Cell Science Volume: 128 Pages: 781-789
  • DOI: 10.1242/jcs.163113
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tricellulin regulates junctional tension of epithelial cells at tricellular contacts through Cdc42. (2014)
  • Author(s): Oda Y, Otani T, Ikenouchi J, Furuse M.
  • Journal Title: Journal of Cell Science Volume: 127 Pages: 4201-12
  • DOI: 10.1242/jcs.150607
  • Peer Reviewed
• [Journal Article] Sphingomyelin clustering is essential for the formation of microvilli. (2013)
  • Author(s): Ikenouchi J, Hirata M, Yonemura S, Umeda M.
  • Journal Title: Journal of Cell Science Volume: 126 Pages: 3585-3592
  • DOI: 10.1242/jcs.122325
  • Peer Reviewed
• [Journal Article] Lipid polarity is maintained in absence of tight junctions. (2012)
  • Author(s): Ikenouchi J, Suzuki M, Umeda K, Ikeda K, Taguchi R, Kobayashi T, Sato SB, Kobayashi T, Stolz DB, Umeda M
  • Journal Title: J Biol Chem Volume: 287(12) Issue: 12 Pages: 9525-33
  • DOI: 10.1074/jbc.m111.327064
  • Peer Reviewed
• [Journal Article] Upregulated function of mitochondria-associated ER membranes in Alzheimer disease (2012)
  • Author(s): Area-Gomez E, Lara Castillo M, Tambini M, Guardia-Laguarta C, Ad J.C. de Groof Madra M, Ikenouchi J, Umeda M, Bird T, Sturley S, and Schon E
  • Journal Title: EMBO J Volume: 31(21) Issue: 21 Pages: 4106-23
  • DOI: 10.1038/emboj.2012.202
  • Peer Reviewed
• [Presentation] 上皮細胞のバリア機能の人為的な制御に向けて (2015)
  • Author(s): 池ノ内 順一
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-25 – 2015-03-28
  • Invited
• [Presentation] 細胞膜脂質による微絨毛形成の制御機構 (2014)
  • Author(s): 池ノ内 順一
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
  • Invited
• [Presentation] Sphingomyelin clustering is essential for the formation of microvilli. (2013)
  • Author(s): 池ノ内順一、米村重信、梅田真郷
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] Elucidation of the roles of membrane lipids in the progression of epithleial-mesenchymal transition (2013)
  • Author(s): Ikenouchi J
  • Organizer: 第3回日仏がん研究ワークショップ
  • Place of Presentation: フランス・トゥールーズ
• [Presentation] 細胞骨格タンパク質による細胞膜脂質の分布・動態の制御機構の解明 (2013)
  • Author(s): 前田 史世、塩見 僚、太田 安隆、池ノ内 順一
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Book] 生体膜の分子機構 リピッドワールドが先導する生命科学 (2014)
  • Author(s): 梅田真郷 （分担執筆：池ノ内順一）
  • Total Pages: 264
  • Publisher: 化学同人
• [Book] 上皮細胞の極性形成とリン脂質：医学のあゆみ「生命を支える脂質―最新の研究と臨床」 (2014)
  • Author(s): 池ノ内 順一
  • Total Pages: 4
  • Publisher: 医歯薬出版株式会社
• [Remarks] 研究室の研究紹介ホームページ
  • URL: http://seibutsu.biology.kyushu-u.ac.jp/~ikenouchi/research%20-%20past2.html
• [Patent(Industrial Property Rights)] タイトジャンクション形成促進剤 (2014)
  • Inventor(s): 池ノ内順一、塩見僚
  • Industrial Property Rights Holder: 国立大学法人 九州大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-083836
  • Filing Date: 2014-04-15