Counter ion movement during Ca2+ release

• Project/Area Number: 24657133
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Kyoto University
• Principal Investigator: TAKESHIMA Hiroshi 京都大学, 薬学研究科(研究院), 教授 (70212024)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 小胞体Ca2+ / カウンターイオン / TRICチャネル / 小胞体Ca2+放出 / 筋小胞体 / Ca2+ストア / Ca2+取り込み / Ca2+ポンプ

Research Abstract

Although counterion movement coupled with Ca2+ release/uptake is probably required for physiologically functioning of intracellular Ca2+ stores, the molecular basis is totally unknown. We recently identified TRIC channels, that likely mediate counter-K+ currents facilitating Ca2+ release. However, TRIC-knockout mice strongly suggest the existence of other channels/transporters mediating counter currents. We attempted to find new candidates for such channels/transporters in this project, and identified Tmem52 and mitsugumin 56 from the muscle sarcoplasmic reticulum.

Research Products

• [Journal Article] Trimeric intracellular cation channels and sarcoplasmic/endoplasmic reticulum calcium homeostasis (2014)
  • Author(s): Zhou, X., Lin, P., Yamazaki, D., Park, K-H., Komazaki, S., Chen, R. S. W., Takeshima, H. & Ma, J.
  • Journal Title: Circ. Res Volume: 114 Pages: 706-716
• [Journal Article] Enhanced dihydropyridine receptor calcium channel activity improves skeletal muscle strength (2013)
  • Author(s): Mosca, B., Bergamelli, L., Vukcevic, M, Lopez, R., Treves, S., Nishi, M., Takeshima, H., Paolini, C., Martini, M., Rispoli, G., Protasi, F. & Zorzato, F.
  • Journal Title: Nat. Commun Volume: 4 Pages: 1541-1541
• [Book] リアノジン受容体を介するチャネル共役による神経機能,脳21(vol. 16) (2013)
  • Author(s): 竹島浩
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/
• [Remarks] 所属研究室ホームページ
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/