Counter ion movement during Ca2+ release

Research Project

Project/Area Number	24657133
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Cell biology
Research Institution	Kyoto University
Principal Investigator	TAKESHIMA Hiroshi 京都大学, 薬学研究科(研究院), 教授 (70212024)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	小胞体Ca2+ / カウンターイオン / TRICチャネル / 小胞体Ca2+放出 / 筋小胞体 / Ca2+ストア / Ca2+取り込み / Ca2+ポンプ
Research Abstract	Although counterion movement coupled with Ca2+ release/uptake is probably required for physiologically functioning of intracellular Ca2+ stores, the molecular basis is totally unknown. We recently identified TRIC channels, that likely mediate counter-K+ currents facilitating Ca2+ release. However, TRIC-knockout mice strongly suggest the existence of other channels/transporters mediating counter currents. We attempted to find new candidates for such channels/transporters in this project, and identified Tmem52 and mitsugumin 56 from the muscle sarcoplasmic reticulum.