| Project/Area Number |
24657142
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SAKAUE Asako 独立行政法人理化学研究所, 細胞機能探索技術開発チーム, 研究員 (90462689)
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| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | DNA methylation / single cell imaging analysis / population analysis / Fucci / cellular heterogeneity / 生物学 / ライブイメージング / DNAメチル化 / 蛍光タンパク質 |
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| Research Abstract |
We developed several genetically encoded probes that monitor DNA methylation states, which we hope will revolutionize our ability to study epigenetic regulation in individual cells. By tuning the affinity of the probe for methylated DNA, we tried to increase the probe response while minimizing the toxicity to recipient cells. Also, we diversified the color of the fluorescent proteins employed in the probe in order to facilitate simultaneous imaging DNA methylation in conjunction with the cell cycle. The microscope system for performing live imaging with a high spatial resolution was built up. We will continue to work on this project in the next Grant-in-Aid for Scientific Research (C) in the H25 fiscal year.
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