| Project/Area Number |
24657143
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
KOBAYASHI Toshihide 独立行政法人理化学研究所, 小林脂質生物学研究室, 主任研究員 (60162004)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 脂質ドメイン / 脂質ラフト / 脂質の非対称性 / 脂質プローブ / コレステロール / スフィンゴミエリン / 脂質結合性毒素 / セカンドメッセンジャー / ジアシルグリセロール / カベオレ / 電子顕微鏡 / スフィンゴ脂質 / 超高解像顕微鏡 |
|---|
| Research Abstract |
Lipid raft is a plasma membrane lipid domain enriched with sphingolipids and cholesterol. Although lipid rafts are involved in various physiological phenomena, the detailed structures of lipid rafts are not well elucidated. We have developed novel protein probes that specifically bind lipid rafts. Using these probes and advanced microscope techniques, we showed that the transbilayer movement of lipid second messenger, diacylglycerol, is regulated by sphingomyelin. Our results also indicate the existence of sphingomyelin-rich membrane domains in both outer and inner leaflet of the plasma membranes from cultured fibroblasts.
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