Development of High sensitive and high-resolution detection of extra-celluar ATP in mouse and searching ATP signal in mouse embryo
Project/Area Number |
24657144
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Developmental biology
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Research Institution | Gunma University |
Principal Investigator |
YAMAMOTO Masamichi 群馬大学, 先端科学研究指導者育成ユニット, 助教 (70423150)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | アデノシン三リン酸 / 細胞外 / アデノシン三リン酸シグナル |
Research Abstract |
To detect the low density and extra-celluar ATP, ATeamL was developed. ATeamL was constructed in FRET-based ATP indicator by genetically linking GFP and Kusabira Orange with epsilon subunit , which was derived from Bacillus (high affinity to ATP). ATeamL, connected to membrane anchoring sequence, was displayed on extra-celluar region in HeLa cell. Now, I generate the transgenic mouse using this construct to be able to detect the extra-celluar ATP in vivo.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] Induction of pulipotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.2014
Author(s)
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Sasaki, A., Yamamoto, M., Nakamura, M., Sutou, K., Osafune, K. and Yamanaka, S.
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Journal Title
Nature Communications
Volume: 5
Issue: 1
Pages: 3678-3678
DOI
Related Report
Peer Reviewed
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