Development of High sensitive and high-resolution detection of extra-celluar ATP in mouse and searching ATP signal in mouse embryo

• Project/Area Number: 24657144
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Gunma University
• Principal Investigator: YAMAMOTO Masamichi 群馬大学, 先端科学研究指導者育成ユニット, 助教 (70423150)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: アデノシン三リン酸 / 細胞外 / アデノシン三リン酸シグナル

Research Abstract

To detect the low density and extra-celluar ATP, ATeamL was developed. ATeamL was constructed in FRET-based ATP indicator by genetically linking GFP and Kusabira Orange with epsilon subunit , which was derived from Bacillus (high affinity to ATP). ATeamL, connected to membrane anchoring sequence, was displayed on extra-celluar region in HeLa cell. Now, I generate the transgenic mouse using this construct to be able to detect the extra-celluar ATP in vivo.

Research Products

• [Journal Article] Induction of pulipotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm. (2014)
  • Author(s): Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Sasaki, A., Yamamoto, M., Nakamura, M., Sutou, K., Osafune, K. and Yamanaka, S.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 3678-3678
  • DOI: 10.1038/ncomms4678
  • Peer Reviewed
• [Journal Article] Spatial restriction of bone morphogenetic protein signaling in mouse gastrula through the mVam2-dependent endocytic pathway (2012)
  • Author(s): Aoyama, Wada et al.
  • Journal Title: Developmental Cell Volume: 22 Issue: 6 Pages: 1163-1175
  • DOI: 10.1016/j.devcel.2012.05.009
  • Peer Reviewed
• [Presentation] マウス発生過程及び心臓を含む疾患におけるATP代謝 (2014)
  • Author(s): 山本正道
  • Organizer: 第3回発生過程におけるエネルギー代謝を考える会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2014-02-14
• [Remarks]
  • URL: http://asrldu.dept.med.gunma-u.ac.jp/myamamoto/masamichi_yamamoto.html
• [Patent(Industrial Property Rights)] ATP可視化動物およびその用途 (2014)
  • Inventor(s): 山本正道
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-005429
  • Filing Date: 2014-01-15
  • Overseas
• [Patent(Industrial Property Rights)] ＡＴＰ可視化動物およびその用途 (2014)
  • Inventor(s): 山本正道
  • Industrial Property Rights Holder: 山本正道
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-005429
  • Filing Date: 2014-01-15