Evolutionary mechanisms of the gastrula organizer and blastopore formation by focusing on the gene regulatory network
| Project/Area Number |
24657147
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAIRA Masanori 東京大学, 理学(系)研究科(研究院), 准教授 (60150083)
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| Co-Investigator(Kenkyū-buntansha) |
三井 優輔 基礎生物学研究所, 大学共同利用機関等の部局等, 助教 (70634129)
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| Project Period (FY) |
2012-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 転写因子 / 分泌性因子 |
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| Research Abstract |
Secreted proteins in the extracellular space and transcription factors functioning in the nuclei do not appear to be related, but they have similarity not only in biochemical characters but also in other features so that some secreted proteins are functioning in the nucleus, and some transcription factors are secreted from the cell. To explore the meaning of these phenomena, we focused on Otx2, which is a transcription factor and reported to be secreted from the cell. We made an Otx2 construct containing a signal peptide and a fluorescent protein, Venus, at the N-terminus, but we could not detect this construct in the extracellular space in the Xenopus embryo. One possibility might be the fact that Otx2 is modified, which we found recently. We revealed that Otx2 is phosphorylated at three serines in the region C-terminal to the homeodomain. Thus, it is important in future to examine whether modifications of Otx2 affect its secretion from the cell.
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Report
(3 results)
Research Products
(10 results)
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[Presentation] Heparan sulfate nanostructures regulate extracellu- lar distribution of Wnt and sFRP2014
Author(s)
Mii, Y., Nakazato, K., Pack, C.-G., Sako, Y., Mochizuki, A., Takada, S. and Taira, M.
Organizer
International Mini Symposium,"Eco-Dev-Evo"
Place of Presentation
京都大学(平良、口頭発表)
Year and Date
2014-02-21
Related Report
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[Presentation] Roles of the zinc finger protein Zbtb11 and phosphorylation modification in the regulation of Otx2 activity in early Xenopus eye development2014
Author(s)
Satou, Y., Hosono, E., Minami, K., Yasuoka, Y., Shibano, T., Mamada, H., Takahashi, S., Asashima, M. and Taira, M.
Organizer
Keystone Symposium on transcription regulation
Place of Presentation
Santa Fe(佐藤、ポスター発表)
Related Report
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[Presentation] Roles of the zinc finger protein Zbtb11 and phosphorylation modification in the regulation of Otx2 activity in early Xenopus eye development.Xenopusの眼の初期形成に関わるOtx2のリン酸化修飾と活性調節におけるZnフィンガー蛋白質Zbtb11の役割2013
Author(s)
Satou, Y., Hosono, E., Minami, K., Shibano, T., Mamada, H., Takahashi, S., Asashima, M. and Taira, M.
Organizer
日本発生生物学会第46回大会
Place of Presentation
松江(佐藤、口頭発表)
Related Report
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[Presentation] Roles of C2H2-type zinc finger proteins, Zbtb11 and Znf668, in Otx2 functions in early Xenopus eye development2012
Author(s)
Satou, Y., Shibano, T., Mamada, H., Takahashi, S., Asashima, M. and Taira, M.
Organizer
14th International Xenopus Conference、Giens Peninsula
Place of Presentation
France
Related Report
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[Presentation] Roles of C2H2-type zinc finger proteins, Zbtb11 and Znf668, in Otx2 functions in early Xenopus eye development2012
Author(s)
Satou, Y., Shibano, T., Mamada, H., Takahashi, S., Asashima, M. and Taira, M.
Organizer
日本発生生物学会第45回大会
Place of Presentation
神戸
Related Report
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