Developmental origin of vertebrate exoskeleton

• Project/Area Number: 24657148
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: Shimada Atsuko 東京大学, 理学(系)研究科(研究院), 助教 (20376552)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: exoskeleton / 外骨格 / 鱗 / 鰭条 / 骨格の起源 / 軟骨魚 / 細胞系譜

Outline of Final Research Achievements

The vertebrate mineralized skeleton is known to have first emerged as an exoskeleton that extensively covered the fossil jawless fish. The evolutionary origin of this exoskeleton has long been attributed to the emergence of the neural crest, but experimental evaluation for this is still poor. Here we tried to develop long-term labeling methods to Dogfish (Scyliorhinus canicula) in order to determine the embryonic origin of ancestral scales. It was found that labeling of somite cells could be possible by injection of DNA with 30 gage needles that are inserted through a thinned eggshell. Next we tried to find the progenitor cells of fins and scales within zebrafish somites and suggested that a small cell population near the notochord could be a candidate progenitor for bone forming cells of the fins and scales.

Research Products

• [Journal Article] Unexpected link between polyketide synthase and calcium carbonate biomineralization (2015)
  • Author(s): Motoki Hojo, Ai Omi, Gen Hamanaka, Kazutoshi Shindo, Atsuko Shimada, Mariko Kondo, Takanori Narita, Masato Kiyomoto, Yohei Katsuyama, Yasuo Ohnishi, Naoki Irie and Hiroyuki Takeda
  • Journal Title: Zoological Letters Volume: 1 Issue: 1 Pages: 1-16
  • DOI: 10.1186/s40851-014-0001-0
  • Peer Reviewed / Open Access
• [Journal Article] Genetic control of startle behavior in medaka fish (2014)
  • Author(s): Tsuboko S, Kimura T, Shinya M, Suehiro Y, Okuyama T, Shimada A, Takeda H, Naruse K, Kubo T, Takeuchi H
  • Journal Title: PLoS one Volume: 9 Issue: 11 Pages: e112527-e112527
  • DOI: 10.1371/journal.pone.0112527
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A neural mechanism underlying mating preferences for familiar individuals in medaka fish. (2014)
  • Author(s): Okuyama T, Yokoi S, Abe H, Isoe Y, Suehiro Y, Imada H, Tanaka M, Kawasaki T, Yuba S, Taniguchi Y, Kamei Y, Okubo K, Shimada A, Naruse K, Takeda H, Oka Y, Kubo T, Takeuchi H
  • Journal Title: Science (New York, N.Y.) Volume: 343 Issue: 6166 Pages: 91-94
  • DOI: 10.1126/science.1244724
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Modular development of the teleost trunk along the dorsoventral axis and zic1/zic4 as selector genes in the dorsal module. (2013)
  • Author(s): Toru Kawanishi
  • Journal Title: Development Volume: 140 Issue: 7 Pages: 1486-1496
  • DOI: 10.1242/dev.088567
  • Peer Reviewed / Open Access
• [Journal Article] The establishment of rotational polarity in the airway and ependymal cilia: analysis with a novel cilium motility mutant mouse (2013)
  • Author(s): Matsuo, M., Shimada, A., Koshida, S., Saga, Y. & Takeda, H.
  • Journal Title: Am J Physiol Lung Cell Mol Physiol Volume: 304 Issue: 11 Pages: L736-L745
  • DOI: 10.1152/ajplung.00425.2012
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Trunk exoskeleton in teleosts is mesodermal in origin. (2013)
  • Author(s): Atsuko Shimada
  • Journal Title: Nature Communications Volume: 4 Issue: 1 Pages: 1-8
  • DOI: 10.1038/ncomms2643
  • Peer Reviewed / Open Access
• [Presentation] 体節細胞の回転によるメダカ体幹部の背腹形成 (2015)
  • Author(s): 島田敦子、田山さやか、西川穂高、武田洋幸
  • Organizer: 第８６回日本動物学会
  • Place of Presentation: 新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2015-09-17
• [Book] 細胞工学 vol32, no8 (2013)
  • Author(s): 島田敦子、武田洋幸
  • Total Pages: 90
  • Publisher: 学研メデイカル秀潤社