| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
In order to develop methods to produce transgenic snails, the techniques of gene transfer into early embryos and of generating expression vectors which can express a foreign gene in germ cells were investigated. With the use of an expression vector containing the endogenous beta-actin promoter sequence, we have succeeded in increasing the survival rate of embryos containing injected exogenous DNA. I-SceI meganuclease method was also applied to obtain the transgenic snails.
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