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Identification of muscle support cells generated during muscledifferentiation

Research Project

Project/Area Number 24657154
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionKyoto University

Principal Investigator

SEHARA Atsuko  京都大学, 再生医科学研究所, 教授 (60209038)

Project Period (FY) 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords筋形成 / 筋衛星細胞 / 幹細胞 / 細胞外基質 / 細胞間相互作用 / 骨格筋 / 筋芽細胞 / MyoD / myogenin / 蛍光タンパク質
Research Abstract

Mechanisms of myogenesis induced by myogenin have been elusive. Wehave succeeded to isolate myogenin-negative cells from differentiating myoblast C2C12cells, and have found that those ells express sets of genes different from myogeninpositive cells by microarray. One of these genes is expressed in regions adjacent tomuscle, and knockdown of this gene significantly affected myogenesis and enhancedapoptosis in zebrafish, supporting an idea that this gene and cells expressing this geneare involved in the maintenance or survival of muscle cells. Whether these cells aregenerated from muscle satellite cells was examined.

Report

(2 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (25 results)

All 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (19 results) (of which Invited: 5 results)

  • [Journal Article] Fetal Skeletal Muscle Progenitors Have Regenerative Capacity After Intramuscular Engraftment in Dystrophin Deficient Mice.2013

    • Author(s)
      Sakai, H.
    • Journal Title

      PLoS ONE

      Volume: in press

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro.2013

    • Author(s)
      Tanaka, A.
    • Journal Title

      PLoS ONE

      Volume: in press

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Activity-dependent proteolytic cleavage of neuroligin-12012

    • Author(s)
      Suzuki K, Hayashi Y, Nakahara S, Kumazaki H, Prox J, Horiuchi K, Zeng M, Tanimura S, Nishiyama Y, Osawa S, Sehara-Fujisawa A, Saftig P, Yokoshima S, Fukuyama T, Matsuki N, Koyama R, Tomita T, Iwatsubo T.
    • Journal Title

      Neuron

      Volume: 76(2) Issue: 2 Pages: 410-422

    • DOI

      10.1016/j.neuron.2012.10.003

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro modeling of paraxial mesodermal progenitors derived from induced pluripotent stem cells.2012

    • Author(s)
      Sakurai, H.
    • Journal Title

      PLoS ONE

      Volume: 7(10) Issue: 10 Pages: e47078-e47078

    • DOI

      10.1371/journal.pone.0047078

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fetal Skeletal MuscleProgenitors Have Regenerative Capacity AfterIntramuscular Engraftment in DystrophinDeficient Mice

    • Author(s)
      Sakai, H., et al
    • Journal Title

      PLoS ONE

      Volume: (in press)

    • Related Report
      2012 Final Research Report
  • [Journal Article] Efficient andreproducible myogenic differentiation fromhuman iPS cells: prospects for modelingMiyoshi Myopathy in vitro

    • Author(s)
      Tanaka, A., et al
    • Journal Title

      PLoS ONE

      Volume: (in press)

    • Related Report
      2012 Final Research Report
  • [Presentation] Exploring roles of ADAMproteases in development using zebrafish2013

    • Author(s)
      Atsuko Sehara
    • Organizer
      国際高等研究所研究プログラム「ゲノム工学とイメージングサイエンスに基づく生命システム研究の新展開」2012年度第1回研究会
    • Place of Presentation
      京都
    • Year and Date
      2013-02-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Exploring Roles ofADAM Proteases in Development usingZebrafish2012

    • Author(s)
      Atsuko Sehara-Fujisawa
    • Organizer
      NUS/TLL/NIBB JOINT PRACTICALWORKSHOP ON Genetics, Genomics & Imaging inMedaka and Zebrafish
    • Place of Presentation
      Singapore,Singapore
    • Year and Date
      2012-07-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 生きたゼブラフィッシュを用いてADAMプロテアーゼの役割と機能に迫る2012

    • Author(s)
      瀬原淳子
    • Organizer
      第12回日本抗加齢医学会総会
    • Place of Presentation
      神奈川
    • Year and Date
      2012-06-24
    • Related Report
      2012 Final Research Report
  • [Presentation] ヘビ毒出血因子のルーツを探る2012

    • Author(s)
      瀬原淳子
    • Organizer
      平成24年度日本生化学会九州支部例会シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2012-05-26
    • Related Report
      2012 Final Research Report
  • [Presentation] The onset of primitive bloodcirculation regurates the timing of bloodvessel2012

    • Author(s)
      Atsuo Iida, Anna Tomosawa, KazuyaSakaguchi, Sigenobu Yonemura, Atsuko SeharaFujisawa
    • Organizer
      Joint Meeting of the BritishSocieties for Cell Biology, DevelopmentalBiology and the Japanese Society forDevelopmental Biology
    • Place of Presentation
      Coventry,UK
    • Year and Date
      2012-04-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Roles of ADAM8 in skeletal muscle regeneration2012

    • Author(s)
      D. Nishimura, H. Sakai, T Sato, F. Sato, J.W. Bartsch, A. Sehara-Fujisawa
    • Organizer
      The American Society for Cell Biology 2012 Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] The onset of primitive blood circulation regurates the timing of blood vessel.

    • Author(s)
      Atsuko Sehara-Fujisawa
    • Organizer
      Joint Meeting of the British Societies for Cell Biology, Developmental Biology and the Japanese Society for Developmental Biology
    • Place of Presentation
      Coventry, UK
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヘビ毒出血因子のルーツを探る

    • Author(s)
      瀬原淳子
    • Organizer
      平成24年度日本生化学会九州支部例会シンポジウム
    • Place of Presentation
      福岡県
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Vascular formation requires crosstalks between endothelial cells and primitver erythrobrasts at the early stage of their development.

    • Author(s)
      Atsuo Iida, Anna Tomosawa, Kazuya Sakaguchi, Daigo Nishimura, Atsuko Sehara-Fujisawa
    • Organizer
      第45回発生生物学会・第64回日本細胞生物学会合同大会
    • Place of Presentation
      兵庫県
    • Related Report
      2012 Annual Research Report
  • [Presentation] ENGRAFTMENT OF MOUSE FETAL MYOGENIC PROGENITORS INTO DYSTROPHIC MUSCLES

    • Author(s)
      Sakai, Hiroshi, Sato, Takahiko, Sakurai, Hidetoshi, Hanaoka,Kazunori, Montarras, Didier, Buckingham, Margaret, Sehara-Fujisawa, Atsuko
    • Organizer
      International Society for Stem Cell Research 10th Annual Meeting
    • Place of Presentation
      神奈川県
    • Related Report
      2012 Annual Research Report
  • [Presentation] ROLES OF ADAM8 FOR SKELETAL MUSCLE REGENERATION

    • Author(s)
      Nishimura, Daigo, Sakai, Hiroshi, Sato, Takahiko, Iida, Atsuo, Sato, Fuminori, Bartsch, Jörg W., Sehara-Fujisawa, Atsuko
    • Organizer
      International Society for Stem Cell Research 10th Annual Meeting
    • Place of Presentation
      神奈川県
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生きたゼブラフィッシュを用いてADAMプロテアーゼの役割と機能に迫る

    • Author(s)
      瀬原淳子
    • Organizer
      第12回日本抗加齢医学会総会
    • Place of Presentation
      神奈川県
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Exploring Roles of ADAM Proteases in Development using Zebrafish

    • Author(s)
      Atsuko Sehara-Fujisawa
    • Organizer
      NUS/TLL/NIBB JOINT PRACTICAL WORKSHOP ON Genetics, Genomics & Imaging in Medaka and Zebrafish
    • Place of Presentation
      Singapore, Singapore
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ゼブラフィッシュの筋維持における増殖因子の役割とその制御

    • Author(s)
      瀬原淳子
    • Organizer
      第26回日本宇宙生物科学会
    • Place of Presentation
      徳島県
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 成体骨格筋幹細胞の形成に関与する転写後調節機構探求

    • Author(s)
      佐藤貴彦、平向洋介、瀬原淳子
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県
    • Related Report
      2012 Annual Research Report
  • [Presentation] デュシェンヌ型筋ジストロフィー患者由来iPS細胞を用いての病態再現

    • Author(s)
      庄子栄美、櫻井英俊、中畑龍俊、田中章仁、ウォルツェン クヌート、藤井宣晴、平家敏男、真鍋康子、瀬原-藤沢淳子
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県
    • Related Report
      2012 Annual Research Report
  • [Presentation] 血液の発生と挙動におけるADAM8の役割

    • Author(s)
      西邨大吾、飯田敦夫、坂口和弥、杉山立樹、長澤丘司、千葉彩乃、望月直樹、バーツ.J.W.、瀬原淳子
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県
    • Related Report
      2012 Annual Research Report
  • [Presentation] 発生過程のゼブラフィッシュ脳の神経細胞の産生におけるErbB4シグナルの役割

    • Author(s)
      佐藤智美、佐藤文規、坂口和弥、谷米竜馬、亀崎青沙、栗崎知浩、瀬原淳子
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県
    • Related Report
      2012 Annual Research Report
  • [Presentation] Exploring roles of ADAM proteases in development using zebrafish

    • Author(s)
      Atsuko Sehara
    • Organizer
      国際高等研究所研究プログラム「ゲノム工学とイメージングサイエンスに基づく生命システム研究の新展開」2012年度第1回研究会
    • Place of Presentation
      京都府
    • Related Report
      2012 Annual Research Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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