Development of newt molecular genetic systems
Project/Area Number |
24657155
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Developmental biology
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Research Institution | Tottori University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
HAYASHI Toshinori 鳥取大学, 医学部, 准教授 (60580925)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | 発生 / 再生 / 有尾両生類 / 分子遺伝学 / トランスジェニック / イベリアトゲイモリ / 近交系 / 純系 |
Research Abstract |
An experimental model system for molecular genetics in newts is necessary to clarify the mechanisms of regeneration from the perspective of gene functions. We have introduced Iberian ribbed newt (Pleurodeles waltl) as the suitable model newts. In the results of our researches, we have been able to shorten the periods for sexual maturation, and are developing 2 inbred and various transgenic lines of P. waltl newts. We also demonstrated that Cre-loxP recombination system efficiently worked in the newts. In addition, we achieved targeted gene knockout in the newt using TALENs. These results show that conditional knockout newts would be available by combination of Cre-loxP recombination and TALENs. Our system would be very powerful for studying the molecular mechanisms of not only regeneration but also various researches. In relation to the result of this research, we published total 12 research and review papers, also our group had 29 conference presentations.
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Report
(3 results)
Research Products
(81 results)
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[Journal Article] Repression of cyclin D1 expression is necessary for the maintenance of cell cycle exit in adult mammalian cardiomyocytes2014
Author(s)
Tane, S., Kubota, M., Okayama, H.,Ikenishi, A., Yoshitome, S., Iwamoto,N., Satoh, Y., Kusakabe, A., Ogawa, S.,Kanai, A., Molkentin, J. D., Nakamura, K., Ohbayashi, T., and Takeuchi, T.
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Journal Title
J. Biol. Chem
Volume: (in press)
Issue: 26
Pages: 18033-18044
DOI
Related Report
Peer Reviewed
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[Journal Article] CDK inhibitors, p21Cip1 and p27Kip1, participate in cell cycle exit of mammalian cardiomyocytes.2014
Author(s)
Tane, S., Ikenishi, A., Okayama, H., Iwamoto, N., Nakayama, I. K., and Takeuchi, T.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 443
Issue: 3
Pages: 1105-1109
DOI
Related Report
Peer Reviewed
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[Journal Article] Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration.2014
Author(s)
Hayashi T, Sakamoto K, Sakuma T, Yokotani N, Inoue T, Kawaguchi E, Agata K, Yamamoto T, Takeuchi T
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Journal Title
Development, Growth and Differentiation
Volume: 56
Issue: 1
Pages: 115-121
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Histone H3 lysine 9 methyltransferases, G9a and GLP are essential for cardiac morphogenesis.2013
Author(s)
Inagawa M, Nakajima K, Makino T, Ogawa S, Kojima M, Ito S, Ikenishi A, Hayashi T, Schwartz RJ, Nakamura K, Obayashi T, Tachibana M, Shinkai Y, Maeda K, Miyagawa-Tomita S, Takeuchi T
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Journal Title
Mech Dev.
Volume: 130
Issue: 11-12
Pages: 519-31
DOI
Related Report
Peer Reviewed
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[Presentation] Molecularmechanisms of cell proliferation in cardiomyocytes during cardiac development and regeneration2014
Author(s)
Takeuchi, T., Tane, S., Okayama,Ikenishi, A., H., Matsumoto, A.,Myouga, A., Hayashi, T.
Organizer
CDB Symposium 2014
Place of Presentation
RIKEN CDB 神戸市
Related Report
Invited
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[Presentation] Molecular genetic system for regenerative studies using newts Iberian Ribbed Newts (Pleurodeles waltl)2014
Author(s)
Hayashi, T., Yokotani, N., Matsumoto,A., Myouga, A., Sakamoto, K., Inoue,T., Kawaguchi, E., Agata, K., Sakuma,T., Yamamoto, T., Takeuchi, T.
Organizer
CDB Symposium 2014
Place of Presentation
RIKEN CDB、神戸市
Related Report
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[Presentation] Molecular mechanisms of cell proliferation in cardiomyocytes during cardiac development and regeneration.2014
Author(s)
Takeuchi, T., Tane, S., Okayama, Ikenishi, A., H., Matsumoto, A., Myouga, A., Hayashi, T.
Organizer
CDB Symposium 2014
Place of Presentation
RIKEN CDB 神戸市
Related Report
Invited
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[Presentation] Molecular genetic system for regenerative studies using newts Iberian Ribbed Newts (Pleurodeles waltl).2014
Author(s)
Hayashi, T., Yokotani, N., Matsumoto, A., Myouga, A., Sakamoto, K., Inoue, T., Kawaguchi, E., Agata, K., Sakuma, T., Yamamoto, T., Takeuchi, T.
Organizer
CDB Symposium 2014
Place of Presentation
RIKEN CDB 神戸市
Related Report
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[Presentation] Molecularmechanisms of cell proliferation in cardiomyocytes during cardiacdevelopment and regeneration2013
Author(s)
Takeuchi, T., Tane, S., Okayama,Ikenishi, A., Yokotani, N., Matsumoto,A., Myouga, A., Hayashi, T.
Organizer
日本発生生物学会
Place of Presentation
くにびきメッセ 松江市
Related Report
Invited
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[Presentation] What molecule triggersre-proliferation of cardiomyocytes during newt heart regeneration?2013
Author(s)
Yokotani, N., Inoue, T., Kawaguchi, E.,Agata, K., Takeuchi, T., Hayashi, T.
Organizer
日本発生生物学会
Place of Presentation
くにびきメッセ 松江市
Related Report
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[Presentation] Molecular genetic systemfor regenerative studies using newt2013
Author(s)
Hayashi, T., Yokotani, N., Matsumoto,A., Myouga, A., Okamoto, M.,Takeuchi, T.
Organizer
日本発生生物学会
Place of Presentation
くにびきメッセ 松江市
Related Report
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[Presentation] Molecular mechanisms of cell proliferation in cardiomyocytes during cardiac development and regeneration.2013
Author(s)
Takeuchi, T., Tane, S., Okayama, Ikenishi, A., Yokotani, N., Matsumoto, A., Myouga, A., Hayashi, T.
Organizer
日本発生生物学会
Place of Presentation
くにびきメッセ 松江市
Related Report
Invited
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[Presentation] Myouga, A., Yokotani, N., Takeuchi, T., Hayashi, T. Linage tracing analysis of cardiomyocytes in the newt cardiac regeneration by tamoxifen-inducible Cre-loxP system.2013
Author(s)
Myouga, A., Yokotani, N., Takeuchi, T., Hayashi, T.
Organizer
日本発生生物学会
Place of Presentation
くにびきメッセ 松江市
Related Report
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[Presentation] What molecule triggers re-proliferation of cardiomyocytes during newt heart regeneration?2013
Author(s)
Yokotani, N., Inoue, T., Kawaguchi, E., Agata, K., Takeuchi, T., Hayashi, T.
Organizer
日本発生生物学会
Place of Presentation
くにびきメッセ 松江市
Related Report
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[Presentation] Molecular genetic system for regenerative studies using newt.2013
Author(s)
Hayashi, T., Yokotani, N., Matsumoto, A., Myouga, A., Okamoto, M., Takeuchi, T.
Organizer
日本発生生物学会
Place of Presentation
くにびきメッセ 松江市
Related Report
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[Presentation] Molecular mechanisms of cell cycle arrest in cardiomyocytes during cardiac development and regeneration2012
Author(s)
Takeuchi, T., Tane, S., Ikenishi, A.,Okayama, H., Yokotani, N.,Matsumoto, A., Hayashi, T.
Organizer
日本分子生物学会 指定演者
Place of Presentation
福岡国際会議場 福岡市
Related Report
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[Presentation] How doadult mammalian cardiomyocytesmaintain the proliferation arrest?2012
Author(s)
Tane, S., Yoshitome, Satoh, Y.,Iwamoto, N., Okayama, H., Ikenishi,A., Hayashi, T., Takeuchi, T.
Organizer
日本分子生物学会
Place of Presentation
福岡国際会議場 福岡市
Related Report
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[Presentation] How does cell cycle ofmouse cardiomyocytes arrest? : analysis of cell cycle regulator2012
Author(s)
Ikenishi, Iwamoto, N., Tane, S.,Okayama, H., Satoh, Y., Hayashi, T.,Takeuchi, T.
Organizer
日本分子生物学会
Place of Presentation
福岡国際会議場 福岡市
Related Report
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[Presentation] How does cell cycle of mouse cardiomyocytes arrest? : analysis of cell cycle pattern2012
Author(s)
Okayama, H., Tane, S., Iwamoto, N.,Ikenishi, A., Satoh, Y., Hayashi, T.,Takeuchi, T.
Organizer
日本分子生物学会
Place of Presentation
福岡国際会議場 福岡市
Related Report
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[Presentation] Molecular geneticsystem for regenerative studiesusing newt2012
Author(s)
Hayashi, T., Yokotani, N., Tane, S.,Matsumoto, A., Myouga, A.,Takeuchi, T.
Organizer
日本分子生物学会
Place of Presentation
福岡国際会議場福岡市
Related Report
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[Presentation] What molecule triggers reproliferationof cardiomyocytesduring newt heart regeneration?2012
Author(s)
Yokotani, N., Matsumoto, A.,Hayashi, T., Inoue, T., Shibata, N.,Kawaguchi, E., Agata, K., Takeuchi,T.
Organizer
日本分子生物学会
Place of Presentation
福岡国際会議場 福岡市
Related Report
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[Presentation] Howdo adult mammalian cardiomyocytesmaintain the proliferation arrest?2012
Author(s)
Tane, S., Yoshitome, Satoh, Y.,Iwamoto, N., Okayama, H., Ikenishi,A., Hayashi, T., Takeuchi, T.
Organizer
日本発生生物学会、細胞生物学会合同大会
Place of Presentation
神戸国際会議場 神戸市
Related Report
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[Presentation] Analysis ofthe expression patterns of Cell cycleregulator genes during newt cardiac egeneration2012
Author(s)
Matsumoto, A., Hayashi, T., Yokotani,N., Kawaguchi, E., Nishimura, O.,Agata, K., Takeuchi, T.
Organizer
日本発生生物学会、細胞生物学会合同大会
Place of Presentation
神戸国際会議場 神戸市
Related Report
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