Analysis of spatiotemporal patterning of WNT proteins that regulate asymmetric patterning

• Project/Area Number: 24657159
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: National Institute of Genetics
• Principal Investigator: IHARA Shinji 国立遺伝学研究所, 構造遺伝学研究センター, 助教 (70373272)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: WNT / 非対称分裂 / 可視化 / Wnt

Research Abstract

It is assumed that a WNT proteins show hydrophilic character based on amino acid sequence. However, these proteins are highly hydrophobic due to adding the lipid and stick tightly to cell membrane and extracellular matrix. To understand spaciotemporal patterning of WNT proteins and extracellular matrix, we use asymmetric division which are regulated by WNT as a model system in C. elegans. We have obtained some results about visualization of WNT molecule and extracellular matrix in vivo.

Research Products

• [Journal Article] In Situ Imaging in C. elegans Reveals Developmental Regulation of Microtubule Dynamics (2014)
  • Author(s): Lacroix B, Bourdages KG, Dorn JF, Ihara S, Sherwood DR, Maddox PS, Maddox AS Dev.
  • Journal Title: cell Volume: 29 Issue: 2 Pages: 203-216
  • DOI: 10.1016/j.devcel.2014.03.007
  • URL: http://www.sciencedirect.com/science/article/pii/S1534580714001592
  • Peer Reviewed
• [Journal Article] The novel secreted factor MIG-18 acts with MIG-17/ADAMTS to control cell migration in Caenorhabditis elegans. (2014)
  • Author(s): Kim H-S, Kitano Y, Mori M, Takano T, Harbaugh TE, Mizutani K, Yanagimoto H, Miwa S, Ihara S, Kubota Y, Shibata Y, Ikenishi K, Garriga G and Nishiwaki K.
  • Journal Title: Genetics Volume: 196(2) Issue: 2 Pages: 471-479
  • DOI: 10.1534/genetics.113.157685
  • URL: http://www.genetics.org/content/196/2/471.long
  • Peer Reviewed / Open Access
• [Journal Article] 線虫における細胞移動・浸潤の制御機構 (2013)
  • Author(s): 伊原伸治
  • Journal Title: 生化学(公社)(日本生化学会出版) Volume: 第85巻第11号(ISSN :0037-1017) Pages: 972-984
  • NAID: 40019893897
• [Journal Article] 線虫における細胞移動・浸潤の制御機構 (2013)
  • Author(s): 伊原伸治
  • Journal Title: 生化学 Volume: 85 Pages: 972-984
  • NAID: 40019893897
• [Presentation] 遺伝学的スクリーニングによって明らかになった糖鎖による恒常性維持機構 (2013)
  • Author(s): 伊原伸治
  • Organizer: 日本糖質学会
  • Place of Presentation: 大阪
  • Year and Date: 2013-08-06
• [Presentation] Analysis of Heparan Sulfate function during C. elegans anchor cell invasion (2013)
  • Author(s): Shinji Ihara, David, R. Sherwood and Sawa Hitoshi
  • Organizer: 日本発生生物学会
  • Place of Presentation: 松江くにびきメッセ
  • Year and Date: 2013-05-30
• [Presentation] 遺伝学的スクリーニングによって明らかとなった糖鎖による恒常性維持機能 (2013)
  • Author(s): 伊原伸治
  • Organizer: 第32回 糖質学会
  • Place of Presentation: 大阪国際交流センター
• [Presentation] Analysis of Heparan Sulfate function during anchor cell invasion in C. elegans (2013)
  • Author(s): Shinji Ihara, David, R. Sherwood and Sawa Hitoshi
  • Organizer: 第46回 日本発生生物学会
  • Place of Presentation: 松江くにびきメッセ
• [Presentation] 細胞移動・浸潤過程を制御する分子の発見と解析 (2012)
  • Author(s): 伊原伸治
  • Organizer: 奨励賞受賞講演第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-14
• [Presentation] Regulation of hole size in basement membrane during cell invasion in C. elegans (2012)
  • Author(s): Ihara, S., David, R. Sherwood, and Sawa, H.
  • Organizer: Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Kobe International Conference Room, JWS-B5 (P1-142)
  • Year and Date: 2012-05-29
• [Presentation] Regulation of hole size in basement membrane during cell invasion in C. elegans (2012)
  • Author(s): Ihara, S., David, R. Sherwood, and Sawa, H.
  • Organizer: East Asia Worm Meeting
  • Place of Presentation: Taipei
• [Presentation] Regulation of hole size in basement membrane during cell invasion in C. elegans. (2012)
  • Author(s): Ihara, S.
  • Organizer: East Asia Worm Meeting
  • Place of Presentation: Taipei, Taiwan
• [Presentation] 細胞移動・浸潤過程を制御する分子の発見と解析
  • Author(s): 伊原伸治
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
  • Invited
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/MultiOrg/Multicellular/Home.html