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Diversity and evolution of gene expression in reptilian mitochondria

Research Project

Project/Area Number 24657163
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Evolutionary biology
Research InstitutionNagoya City University

Principal Investigator

KUMAZAWA Yoshinori  名古屋市立大学, 大学院システム自然科学研究科, 教授 (60221941)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsミトコンドリア / RNA-Seq / 爬虫類 / 次世代シーケンス / RNA編集 / polyA付加 / RNA / 遺伝子発現 / polyA / 次世代シーケンサー / トランスクリプトーム / ミトコンドリアDNA / 翻訳異常 / 遺伝子配置変動
Research Abstract

We conducted RNA-Seq experiments using animal species representing Reptilia. We found some variations in the distribution of polyA-addition sites to mRNAs and some of them are consistent with variations of gene arrangements of mtDNAs. Accuracy of polyA addition was lowered to some extent for particular mRNAs in a species though reasons for this are currently unknown. Relative ratio of each mRNA abundance in a species is changed in a way that is consistent with an idea that polycistronic mRNAs are created from one control region to another. The gene expression system in mitochondria has variations and is evolving in Reptilia.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (22 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (17 results) (of which Invited: 2 results)

  • [Journal Article] Molecular phylogeny and biogeography of air sac catfishes of the Heteropneustes fossilis species complex (Siluriformes: Heteropneustidae)2014

    • Author(s)
      Ratmuangkhwang, S., Musikasinthorn, P., Kumazawa, Y.
    • Journal Title

      Molecular Phylogenetics and Evolution

      Volume: ** Pages: 82-91

    • DOI

      10.1016/j.ympev.2014.05.009

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 脊椎動物嗅覚受容体遺伝子ファミリーの進化研究における次世代シーケンサーの活用2013

    • Author(s)
      橋口康之、熊澤慶伯
    • Journal Title

      生物科学

      Volume: 64 Pages: 131-140

    • URL

      http://www.ruralnet.or.jp/seibutsu/064_03.htm

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of squamate olfactory receptor genes and their transcripts by the high-throughput sequencing approach2012

    • Author(s)
      Dehara Y, Hashiguchi Y, Matsubara K, Yanai T, Kubo M and Kumazawa Y
    • Journal Title

      Genome Biology and Evolution

      Volume: 4(4) Issue: 4 Pages: 602-616

    • DOI

      10.1093/gbe/evs041

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial genomes of two African geckos of genus Hemitheconyx(Squamata : Eublepharidae)2012

    • Author(s)
      Jonniaux P and Kumazawa Y
    • Journal Title

      Mitochondrial DNA

      Volume: (印刷中) Issue: 4 Pages: 278-279

    • DOI

      10.3109/19401736.2012.668898

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Intra-genomic GC heterogeneity in sauropsids : evolutionary insights from cDNA mapping and GC_3 profiling in snake2012

    • Author(s)
      Matsubara K, Kuraku S, Tarui H, Nishimura O, Nishida C, Agata K, Kumazawa Y, Matsuda Y
    • Journal Title

      BMC Genomics

      Volume: 13 Issue: 1 Pages: 604-604

    • DOI

      10.1186/1471-2164-13-604

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] RNA-Seqデータから脊椎動物の分子系統解析に繋げるためのパイプラインの構築2013

    • Author(s)
      栗崎政希、Pierre Jonniaux、松原和純、熊澤慶伯
    • Organizer
      日本進化学会第15回大会
    • Place of Presentation
      筑波大学
    • Year and Date
      2013-08-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 爬虫類ミトコンドリアRNAの構造多様性の網羅的探索2013

    • Author(s)
      孫ぎょう、栗崎政希、熊澤慶伯
    • Organizer
      日本進化学会第15回大会
    • Place of Presentation
      筑波大学
    • Year and Date
      2013-08-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 爬虫類ミトコンドリアにおける転写物の網羅的解析2013

    • Author(s)
      孫ぎょう、栗崎政希、熊澤慶伯
    • Organizer
      日本進化学会第14回大会
    • Place of Presentation
      首都大学東京
    • Year and Date
      2013-08-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 爬虫類ミトコンドリアRNAの構造多様性の網羅的探索2013

    • Author(s)
      孫ぎょう、栗崎正希、熊澤慶伯
    • Organizer
      日本進化学会第15回大会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] RNA-Seqデータから脊椎動物の分子系統解析に繋げるためのパイプラインの構築2013

    • Author(s)
      栗崎政希、Pierre Jonniaux、松原和純、熊澤慶伯
    • Organizer
      日本進化学会第15回大会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヤモリ類のミトコンドリアゲノミクス : 遺伝子配置変動と系統関係2012

    • Author(s)
      熊澤慶伯、三浦沙綾、孫ぎょう、ピエールジョニオ、山田知江美、橋口康之
    • Organizer
      日本分子生物学会第35回大会
    • Place of Presentation
      福岡国際会議場&マリンメッセ博多
    • Year and Date
      2012-12-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 尾張地区におけるカスミサンショウウオの遺伝的解析2012

    • Author(s)
      藤谷武史、能登原盛弘、熊澤慶伯
    • Organizer
      日本爬虫両棲類学会第51回大会
    • Place of Presentation
      愛知学泉大学
    • Year and Date
      2012-11-10
    • Related Report
      2013 Final Research Report
  • [Presentation] カメ類ミトコンドリアゲノムにおける翻訳フレームシフトの分子進化2012

    • Author(s)
      山田知江美、柴田弘紀、熊澤慶伯
    • Organizer
      日本進化学会第14回大会
    • Place of Presentation
      首都大学東京
    • Year and Date
      2012-08-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Characteristics of mitogenomic data for vertebrate phylogenetics2012

    • Author(s)
      Kumazawa, Y., Kurisaki, M., and Sun, Y.
    • Organizer
      The Plenary International Symposium "Phylogenetics", Society of Evolutionary Studies, Japan
    • Place of Presentation
      首都大学東京
    • Year and Date
      2012-08-22
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] トランスクリプトームを用いたヘビ類の系統的位置付けの解析2012

    • Author(s)
      栗崎政希、松原和純、熊澤慶伯
    • Organizer
      日本進化学会第14回大会
    • Place of Presentation
      首都大学東京
    • Year and Date
      2012-08-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Characteristics of mitogenomic data for vertebrate phylogenetics2012

    • Author(s)
      Kumazawa, Y. Kurisaki, M. and Sun, Y.
    • Organizer
      The Plenary International Symposium "Phylogenetics", Society of Evolutionary Studies, Japan
    • Place of Presentation
      Tokyo Metropolitan University, Hachioji City
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] トランスクリプトームを用いたヘビ類の系統的位置付けの解析2012

    • Author(s)
      栗崎政希、松原和純、熊澤慶伯
    • Organizer
      日本進化学会第14回大会
    • Place of Presentation
      首都大学東京(八王子市)
    • Related Report
      2012 Research-status Report
  • [Presentation] カメ類ミトコンドリアゲノムにおける翻訳フレームシフトの分子進化2012

    • Author(s)
      山田知江美、柴田弘紀、熊澤慶伯
    • Organizer
      日本進化学会第14回大会
    • Place of Presentation
      首都大学東京(八王子市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 並列DNAシーケンサーを用いた有鱗類嗅覚受容体遺伝子群の網羅的解析2012

    • Author(s)
      橋口康之、出原由季、松原和純、柳井徳磨、久保正仁、熊澤慶伯
    • Organizer
      日本進化学会第14回大会
    • Place of Presentation
      首都大学東京(八王子市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 爬虫類ミトコンドリアにおける転写物の網羅的解析2012

    • Author(s)
      孫ぎょう、栗崎正希、熊澤慶伯
    • Organizer
      日本進化学会第14回大会
    • Place of Presentation
      首都大学東京(八王子市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 尾張地区におけるカスミサンショウウオの遺伝的解析2012

    • Author(s)
      藤谷武史、能登原盛弘、熊澤慶伯
    • Organizer
      日本爬虫両棲類学会第51回大会
    • Place of Presentation
      愛知学泉大学(豊田市)
    • Related Report
      2012 Research-status Report
  • [Presentation] ヤモリ類のミトコンドリアゲノミクス:遺伝子配置変動と系統関係2012

    • Author(s)
      熊澤慶伯、三浦沙綾、孫ぎょう、ピエールジョニオ、山田知江美、橋口康之
    • Organizer
      日本分子生物学会第35回大会
    • Place of Presentation
      福岡国際会議場&マリンメッセ(博多)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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