| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Ascidian can produce normal-looking miniature tailbud embryos (dwarf embryo) even if unfertilized egg was partially removed. By using this dwarf embryo, we investigated how egg volume influenced on the afterward development at single cell level. As a result, it was found that several tissues (notochord, muscle and endoderm) kept the number of cells in dwarf embryo. On the other hand, other tissues (epidermis, neuron cells and mesenchyme) kept the size of each cell. However, the volume ratio of each tissue in whole-volume was same among dwarf and WT embryo. It was suggested that there is a robust mechanism to maintain a constant ratio of tissue volume regardless of egg volume.
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