Development of a system of enhanced plant growth by gene transfection of the fastest myosin.
| Project/Area Number |
24658002
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | Chiba University |
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Principal Investigator |
ITO Kohji 千葉大学, 理学(系)研究科(研究院), 准教授 (50302526)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | シロイヌナズナ / 車軸藻 / 原形質流動 / ミオシン / イネ / アクチン |
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| Research Abstract |
Cytoplasmic streaming is active transport widely occurred in plant cells. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin-XI sliding along actin cables, the fundamental function in plants remains unknown. We generated high-speed chimeric myosin XI by replacing the motor domain of Arabidopsis thaliana myosin XI-2 with that of Chara corallina myosin. Surprisingly, the plant size of the transgenic Arabidopsis high speed chimeric myosin XI-2 was higher than that that of the wild-type plant. This size change correlated with acceleration of cytoplasmic streaming. These results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.
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Report
(3 results)
Research Products
(32 results)
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[Journal Article] Rapid nucleotide exchange renders Asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivo.2012
Author(s)
Umeki, N., Nakajima, J., Noguchi, T.Q.P., Tokuraku, K., Nagasaki, A., Ito, K., Hirose, K. and Uyeda, T.Q.P.
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Journal Title
J. Biol. Chem.
Volume: 288
Issue: 3
Pages: 1739-1748
DOI
Related Report
Peer Reviewed
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[Journal Article] G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility.2012
Author(s)
Noguchi, T. Q. P., Komori, T., Umeki, N., Demizu, N., Ito, K., Iwane, A. H., Tokuraku, K., Yanagida, T., Uyeda, T. Q. P.
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Journal Title
J. Biol. Chem.
Volume: 279
Issue: 29
Pages: 24399-34345
DOI
Related Report
Peer Reviewed
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[Presentation] ミオシンXIの1分子解析2012
Author(s)
森川高光,岩城光宏,伊藤光三,木村篤,富永基樹,池崎圭吾,小森智貴,藤田恵介,中野明彦,山本啓一,柳田敏雄
Organizer
日本植物学会第76回大会
Place of Presentation
兵庫県立大学
Year and Date
2012-09-15
Related Report
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[Presentation] 分子レベルから眺める原形質流動2012
Author(s)
富永基樹,伊藤光三,小嶋寛明,横田悦雄,山本啓一,新免輝男,大岩和弘,中野明彦
Organizer
日本植物学会第76回大会
Place of Presentation
兵庫県立大学
Year and Date
2012-09-15
Related Report
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[Presentation] ミオシンXI の1 分子解析2012
Author(s)
森川 高光, 岩城 光宏, 伊藤 光二, 木村 篤, 富永基樹, 池崎 圭吾, 小森 智貴, 藤田 恵介, 中野明彦, 山本 啓一, 柳田 敏雄
Organizer
日本植物学会第76回大会
Place of Presentation
兵庫県立大学姫路書写キャンパス
Related Report
Invited
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[Presentation] 分子レベルから眺める原形質流動2012
Author(s)
富永 基樹, 伊藤 光二, 小嶋 寛明, 横田 悦雄, 山本 啓一, 新免 輝男, 大岩 和弘, 中野 明彦
Organizer
日本植物学会第76回大会
Place of Presentation
兵庫県立大学姫路書写キャンパス
Related Report
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