| Project/Area Number |
24658004
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
YAMADA TETSUYA 東京農工大学, (連合)農学研究科(研究院), 准教授 (20422511)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 種間雑種 / 雑種致死 / 克服 / DNAメチル化 / オートファジー / タバコ / 交雑障壁 / 生殖的隔離 / 次世代シーケンサー / 比較トランスクリプトーム / miRNA |
|---|
| Research Abstract |
In this study, in order to obtain new knowledge that leads to solve the problem of hybrid lethality, which is a type of hybridization barriers, some experiments using cultured cells from interspecific hybrids (Nicotiana suaveolens x N. tabacum) are performed and the following results are obtained. 1) Expression levels of autophagy-related genes were increased in hybrid cells before showing the lethality. 2) It was confirmed that mutant cells not showing the lethality were occurred at a high frequency in hybrid cells. DNA methylation level slightly higher than the parental cells was detected in hybrid cells overcame the lethality. Treatment with de novo methylation inhibitor was induced temperature sensitive cell death to hybrid cells overcame the lethality. These results suggest that DNA methylation is involved in overcoming hybrid lethality.
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