Development of a high-efficient gene targeting technique

• Project/Area Number: 24658005
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Okayama University
• Principal Investigator: MURATA MINORU 岡山大学, その他部局等, 教授 (20166292)
• Co-Investigator(Renkei-kenkyūsha): KASHIHARA Kazunari 岡山大学, 資源植物科学研究所, 技術専門職員 (60379807)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 遺伝子ターゲッティング / 染色体工学 / Cre/LoxP / 相同組み換え / ターゲッティング / シロイヌナズナ / GFP

Research Abstract

To aim at establishing a high-efficient homologous recombination system in higher plants, we investigated a relationship between occurrence of circularized T-DNA and transformation efficiency. A co-infection with a target construct and a Cre-expressing construct to Arabidopsis seedlings was found to be effective in the circularization of T-DNA and integration into chromosomes.

Research Products

• [Journal Article] Minichromosomes and artificial chromosomes in Arabidopsis. (2014)
  • Author(s): Murata, M.
  • Journal Title: Chromosome Research Volume: In press Issue: 2 Pages: 167-178
  • DOI: 10.1007/s10577-014-9421-0
  • Peer Reviewed / Open Access
• [Presentation] シロイヌナズナにおけるミニ染色体への遺伝子導入 (2013)
  • Author(s): 村田稔・横田悦子・柴田洋・金谷麻加・藤本聡・柏原壱成・長岐清孝
  • Organizer: 染色体学会第64回年会
  • Place of Presentation: 富山市
• [Presentation] シロイヌナズナおける染色体再編成の誘発とゲノム安定性 (2013)
  • Author(s): 村田稔・柴田洋・藤本聡・長岐清孝
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 福岡市
  • Invited
• [Presentation] シロイヌナズナにおける染色体再編成の誘発とゲノム安定性 (2012)
  • Author(s): 村田稔・柴田洋・藤本聡・長岐清孝
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 福岡市
• [Remarks]
  • URL: http://www.rib.okayama-u.ac.jp/nucleus/index-j.html
• [Remarks] 核機能分子解析グループホームページ
  • URL: http://www.rib.okayama-u.ac.jp/nucleus/index-j.html