Development of a high-efficient gene targeting technique
Project/Area Number |
24658005
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Breeding science
|
Research Institution | Okayama University |
Principal Investigator |
MURATA MINORU 岡山大学, その他部局等, 教授 (20166292)
|
Co-Investigator(Renkei-kenkyūsha) |
KASHIHARA Kazunari 岡山大学, 資源植物科学研究所, 技術専門職員 (60379807)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 遺伝子ターゲッティング / 染色体工学 / Cre/LoxP / 相同組み換え / ターゲッティング / シロイヌナズナ / GFP |
Research Abstract |
To aim at establishing a high-efficient homologous recombination system in higher plants, we investigated a relationship between occurrence of circularized T-DNA and transformation efficiency. A co-infection with a target construct and a Cre-expressing construct to Arabidopsis seedlings was found to be effective in the circularization of T-DNA and integration into chromosomes.
|
Report
(3 results)
Research Products
(6 results)