Regulation of root nodule formation by control of light quality and its application for legume production

• Project/Area Number: 24658020
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Crop science/Weed science
• Research Institution: Saga University
• Principal Investigator: SUZUKI Akihiro 佐賀大学, 農学部, 教授 (50305108)
• Co-Investigator(Kenkyū-buntansha): ARIMA Susumu 佐賀大学, 農学部, 教授 (90140954)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ダイズ / 根粒 / 根粒菌 / 窒素固定 / 共生 / 子実生産 / 収量 / 光 / 遠赤色光 / 光質

Research Abstract

Light is critical for supplying carbon to the energetically expensive, nitrogen-fixing symbiosis between legumes and rhizobia. I have already showed that root nodule formation is photomorphogenetically controlled by sensing the red/far red (R/FR) ratio through JA signaling. In this study, I found that condition of light treatment for increase of the number of root nodule of soybean. As expected, when three day-old soybean plants were treated by FR for 3 weeks, root nodule number was significantly decreased compared with non-treated control plants. However, root nodule number was significantly increased by 3 weeks FR treatment plus 4 weeks natural sunlight treatment compared with non-treated soybean. If this light treatment is applied for soybean production, there is a possibility that soybean yield increase due to the enhancement of root nodule nitrogen fixation by the symbiosis with Bradyrhizobium japonicum.

Research Products

• [Journal Article] 光質が根粒菌の感染を制御するーフィトクロムシグナリングを介した根粒形成のメカニズムー (2014)
  • Author(s): 永田真紀,鈴木章弘
  • Journal Title: 化学と生物 Volume: 52巻 Pages: 136-138
  • NAID: 130004808952
  • Peer Reviewed
• [Journal Article] 光質が根粒菌の感染を制御するーフィトクロムシグナリングを介した根粒形成のメカニズムー (2014)
  • Author(s): 永田真紀，鈴木章弘
  • Journal Title: 化学と生物 Volume: 52 Pages: 136-138
  • NAID: 130004808952
  • Peer Reviewed
• [Journal Article] Additional cause for reduced JA-Ile in the root of a Lotus japonicus phyB mutant (2012)
  • Author(s): Shigeyama T, Tominaga A, Arima S, Sakai T, Inada , Jikumaru , Kamiya , Uchiumi , Abe M, Hashiguchi M, Akashi R, Hirsch AM, and Suzuki A, Shigeyama T, Tominaga A, Arima S, Sakai T, Inada , Jikumaru , Kamiya , Uchiumi , Abe M, Hashiguchi M, Akashi R, Hirsch AM, and Suzuki A
  • Journal Title: Plant Signal Behav. Volume: 7 Issue: 7 Pages: 1-3
  • DOI: 10.4161/psb.20407
  • Peer Reviewed
• [Presentation] Both root nodulation and arbuscular mycorrhization are photomorphogeneticall controlled by sensing the red/far red (R/FR) ratio through jasmonic acid (JA) signaling (2013)
  • Author(s): Akihiro Suzuki et al
  • Organizer: 18^<th> International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Effectt of light irradiation to the root on Lotus japonicus root nodule formation (2013)
  • Author(s): Aya Shimomura, Akihiro Suzuki et al
  • Organizer: 18^<th> International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Effect of the diversity of SEN1 gene on nitrogen fixation activity in Lotus japonicus (2013)
  • Author(s): Katsuya Harada, Akihiro Suzuki et al
  • Organizer: 18^<th> International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Transcriptional analysis of an in vitro nitrogen fixation reaction induced by free living bacterial cells of B. japonicum USDA110 with symbiosome solution of soybean root nodules (2013)
  • Author(s): Tadashi Yokoyama, Akihiro Suzuki et al
  • Organizer: 18^<th> International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Control of mycorrhizal infection by Red/Far-Red (R/FR) ratio through jasmonic acid (JA) signaling (2013)
  • Author(s): Akihiro Suzuki et al
  • Organizer: Plant Biology 2013
  • Place of Presentation: Providence (USA)
• [Presentation] Both root nodulation and arbuscular mycorrhization are photomorphogenetically controlled by sensing the red/far red (R/FR) ration through jasmonic acid (JA) signaling. (2013)
  • Author(s): Akihiro Suzuki et al.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Effect of light irradiation to the root on Lotus japonicas root nodule formation (2013)
  • Author(s): Aya Shimomura et al.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Effect of the diversity of SEN1 gene on nitrogen fixation activity in Lotus japonicus (2013)
  • Author(s): Katsuya Harada et al.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Transcriptional analysis of an in vitro nitrogen fixation reaction induced by free living bacterial cells of B. japonicum USDA110 with symbio (2013)
  • Author(s): Tadashi Yokoyama et al.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Control of mycorrhizal infection by Red/Far-Red (R/FR) ratio through jasmonic acid (JA) signaling. (2013)
  • Author(s): Akihiro Suzuki et al.
  • Organizer: Plant Biology 2013
  • Place of Presentation: Providence
• [Presentation] 高等植物への菌根菌感染は光の質と量によって制御される (2013)
  • Author(s): 鈴木章弘等
  • Organizer: 日本作物学会第235回講演会
  • Place of Presentation: 川崎市
• [Presentation] A possible role of vitamin B6 metabolic pathway of Mesorhizobium loti in symbiosis with Lotus japonicus. (2012)
  • Author(s): A. Suzuki et al.
  • Organizer: 第１０回ヨーロッパ窒素固定会議
  • Place of Presentation: ミュンヘン（ドイツ）
• [Presentation] Expression of b-1,3-glucanase gene in autoregulation of nodulation (2012)
  • Author(s): K. Osuki et al.
  • Organizer: 第２回アジア窒素固定会議
  • Place of Presentation: プーケット（タイ）
• [Presentation] Characterization of LjGlu1, b-1,3-glucanase gene of Lotus japonicus. (2012)
  • Author(s): S. Hashimoto et al.
  • Organizer: 第２回アジア窒素固定会議
  • Place of Presentation: プーケット（タイ）
• [Presentation] Molecular mechanism of improved symbiotic phenotype in ABA low-sensitive mutant enf1 of Lotus japonicus. (2012)
  • Author(s): A. Tominaga et al.
  • Organizer: 第２回アジア窒素固定会議
  • Place of Presentation: プーケット（タイ）
• [Presentation] Low R/FR decreased mycorrhizal infection in higher plants. (2012)
  • Author(s): M. Nagata et al.
  • Organizer: 第２回アジア窒素固定会議
  • Place of Presentation: プーケット（タイ）
• [Book] Chapter 6 of"Advences in Biology and Ecology of Nitrogen Fixation": Effects of Phytohormones on Nodulation and Nitrogen Fixation in Leguminous Plants (2014)
  • Author(s): Maki Nagata,Akihiro Suzuki
  • Publisher: INTECH
• [Book] Chapter 57 of"Biological Nitrogen Fixation": Lotus japonicus nodules when it sees red (2014)
  • Author(s): Maki Nagata,Ann M. Hirsch,Akihiro Suzuki
  • Publisher: Wiley/Blackwell(in press)
• [Book] Chapter 6 of "Advances in Biology and Ecology of Nitrogen Fixation" : Effects of Phytohormones on Nodulation and Nitrogen Fixation in Leguminous Plants (2014)
  • Author(s): Maki Nagata and Akihiro Suzuki
  • Publisher: INTECH
• [Book] Chapter 57 of "Biological Nitrogen Fixation" : Lotus japonicus nodules when it sees red. (2014)
  • Author(s): Maki Nagata, Ann M. Hirsch and Akihiro Suzuki
  • Publisher: Wiley/Blackwell
• [Remarks]
  • URL: http://extwww.cc.saga-u.ac.jp/~azuki/top.html
• [Remarks] 佐賀大学農学部生物環境科学科作物生態生理学研究室ホームページ
  • URL: http://extwww.cc.saga-u.ac.jp/&#771;azuki/top.html
• [Remarks] 佐賀大学農学部生物環境科学科作物生態生理学研究室ホームページ
  • URL: http://extwww.cc.saga-u.ac.jp/~azuki/top.html