Study on rhizobial infection to the cluster roots of Lupinus species and its application to the inoculation practices

• Project/Area Number: 24658021
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Crop science/Weed science
• Research Institution: Osaka Prefecture University
• Principal Investigator: DAIMON Hiroyuki 大阪府立大学, 生命環境科学研究科(系), 教授 (50236783)
• Co-Investigator(Kenkyū-buntansha): MATSUMURA Atsushi 大阪府立大学, 大学院生命環境科学研究科, 助教 (30463269) ; IHARA HIdeshi 大阪府立大学, 大学院理学系研究科, 准教授 (60254447)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 飼料作物 / 窒素固定 / 根系発育 / 地力増強 / フラボノイド / 根粒 / リン酸 / 作物生産科学 / 作物学

Outline of Final Research Achievements

Lupinus species are temperate legume showing positive effects on soil fertility due to the specific root growth under low P condition. The genus Lupinus includes 300 species spread over the world. We investigated the interspecific differences in cluster root formation, exudation of carboxylates, and root nodule formation among these species. Some species from the Old and New World were compared to understand the adaptation to low P. L. mutabilis, which is originated from South America, did not produce the cluster root, but L. albus from the Mediterranean area showed it. Carboxylates exuduated from roots were mainly citrate and malate. Several bacterial isolates from root nodules were clarified to Bradyrhizobium, Rhizobium, and Pseudomonas. Luteolin was not effective for bacteria proliferation. To define the infection process of the bacteria, a gusA-marker gene was transferred to the isolates. The further research on rhizobial infection to the cluster root is now in progress.

Research Products

• [Journal Article] Formation of densely branched lateral roots in Sesbania cannabina triggered by patchily distributed phosphorus in andosolic soils (2015)
  • Author(s): Yuri Funakoshi, Hiroyuki Daimon and Atsushi Matsumura
  • Journal Title: Plant Root Volume: 9
  • NAID: 130005073313
  • Peer Reviewed
• [Journal Article] 植物における根の形態と機能の科学 (2014)
  • Author(s): 大門弘幸
  • Journal Title: 機能材料 Volume: 34 Pages: 55-57
• [Journal Article] Possibility of Introducing Winter Legumes, Hairy Vetch and Faba Bean, as Green Manures to Turmeric Cropping in Temperate Region (2014)
  • Author(s): Yamawaki, K., Matsumura, A., Hattori, R., Tarui, A., Hossain, M. A., Ohashi, Y., and Daimon, H.
  • Journal Title: Plant Production Science Volume: 17 Issue: 2 Pages: 173-184
  • DOI: 10.1626/pps.17.173
  • NAID: 130004462321
  • ISSN: 1343-943X, 1349-1008
  • Peer Reviewed
• [Journal Article] Enhancement of nitrogen uptake in oat by cutting hairy vetch grown as an associated crop (2013)
  • Author(s): Tarui, A., Matsumura, A., Asakura, S., Yamawaki, K., Hattori, R. and Daimon, H.
  • Journal Title: Plant Root Volume: 7 Issue: 0 Pages: 83-91
  • DOI: 10.3117/plantroot.7.83
  • NAID: 130003384306
  • ISSN: 1881-6754
  • Peer Reviewed
• [Journal Article] Evaluation of Mixed Cropping of Oat and Hairy Vetch as Green Manure for Succeeding Corn Production (2013)
  • Author(s): Tarui, A., Matsumura, A., Asakura, S., Yamawaki, K., Hattori, R. and Daimon, H.
  • Journal Title: Plant Production Science Volume: 16 Issue: 4 Pages: 383-392
  • DOI: 10.1626/pps.16.383
  • NAID: 130004850102
  • ISSN: 1343-943X, 1349-1008
  • Peer Reviewed
• [Journal Article] Plant regeneration from <i>Crotalaria spectabilis</i> hairy roots which showed inhibited growth of root-knot nematodes (<i>Meloidogyne hapla</i> and <i>M. incognita</i>) <i>in vitro</i> (2012)
  • Author(s): Ohara, A. et al
  • Journal Title: Plant Biotechnology Volume: 29 Issue: 5 Pages: 425-430
  • DOI: 10.5511/plantbiotechnology.12.0718a
  • NAID: 130002500894
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Presentation] Improving Legume Productivity under Suboptimal Environmental Conditions (2015)
  • Author(s): Hiroyuki Daimon
  • Organizer: Conference of Acian Pacific Chemical, Biological, Environmental Engineering Sciences
  • Place of Presentation: 京都市
  • Year and Date: 2015-04-06 – 2015-04-07
  • Invited
• [Presentation] 黒ボク土におけるリン酸施用がSesbania cannabinaの根系構造に及ぼす影響 (2013)
  • Author(s): 船越有里・大門弘幸・松村篤
  • Organizer: 根研究学会
  • Place of Presentation: 九州沖縄農研センター（鹿児島）
• [Presentation] ラッカセイ－コムギ－緑肥作物の作付におけるアーバスキュラー菌根菌の動態 (2013)
  • Author(s): 松村篤・大門弘幸
  • Organizer: 根研究学会
  • Place of Presentation: 九州沖縄農研センター（鹿児島）
• [Presentation] 沖縄県宮古島特産の黒小豆（ササゲ）の生産と利用の現状 (2013)
  • Author(s): 大橋 善之・松村 篤・今堀 義洋・大門 弘幸
  • Organizer: 日本作物学会
  • Place of Presentation: 鹿児島大学（鹿児島）
• [Presentation] Lupinus属植物4種の低リン条件に対する応答反応 (2012)
  • Author(s): 江川 明日香 ら
  • Organizer: 第37回根研究集会
  • Place of Presentation: 京都大学理学部， 京都市
• [Presentation] Influence of excess soil water during early growth stage on growth, nutrient uptake, and yield of soybean and AM colonization and biomass (2012)
  • Author(s): Hattori, R. et al.
  • Organizer: 第6回国際作物学会議
  • Place of Presentation: ブラジル国， ベントゴンザルベス市
• [Presentation] Morphological changes in roots of Pisum sativum L. inoculated with preincubated rhizobia with naringenin (2012)
  • Author(s): Nakamura, S. et al.
  • Organizer: 第6回国際作物学会議
  • Place of Presentation: ブラジル国， ベントゴンザルベス市
• [Presentation] Cutting the aboveground parts of hairy vetch enhances N uptake of the mixed-cropped oat grown as green manures (2012)
  • Author(s): Tarui, A. et al.
  • Organizer: 第6回国際作物学会議
  • Place of Presentation: ブラジル国， ベントゴンザルベス市