| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this experiment, we determined the distribution of chrysanthemum stunt viroid in chrysanthemum cells and from this result it was revealed that CSVd is suitable as model RNA molecules for screening of proteins which helps trafficking of RNA. And transgenic plants with over-expression of partial CSVd sequences were generated. These plants can be used for the determination of important sequences for traffic CSVd RNA to nucleus and this information is valuable for screening of trafficking proteins with CSVd. And we compared RNA expression in CSVd-infected and -non infected tobacco plants and could screened 8 genes of candidates for trafficking proteins. Now these genes are under being analyzed in detail. In addition, we developed the protein-screening method using chrysanthemum plants, which are strong resistant to CSVd. The transgenic plants over expressed of CSVd in these resistant cultivars were prepared here.
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