Development of an evaluation system for inducibility of strawberry allergy.

• Project/Area Number: 24658030
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kobe University
• Principal Investigator: UNO Yuichi 神戸大学, (連合)農学研究科(研究院), 准教授 (90304120)
• Co-Investigator(Kenkyū-buntansha): NABE Takeshi 京都薬科大学, 薬学部, 准教授 (40228078) ; NITTA Yoko 岡山県立大学, 保健福祉学部, 准教授 (70403318) ; TSURUTA Hiroki 神戸大学, 連携創造本部, 准教授 (20346282)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: イチゴ / バラ科果実 / 品種間差 / 栽培方法 / シラカバ花粉症 / 口腔アレルギー症候群 / バラ科果樹

Research Abstract

Fruit allergies are serious health problems that limit the quality of life and slumping consumption of agricultural products. Strawberry can cause oral allergy syndrome, an allergic reaction in patients. In this study, the fundamental knowledge was obtained by analyzing strawberry allergen Fra a and antiallergic constituents, towards the development of an evaluation system for inducibility of strawberry allergy. The analysis of IgE binding capacity revealed that Fra a 1 might plays an important role in determining the allergenicity of strawberry fruits. Fra a 1 content varied by cultivars, cultivating methods, ripening stages, and different tissues. The secondary structure of Fra a 1 was altered by heating to more than 60 C. There is a difference in antiallergic effects among strawberry cultivars. These findings suggest that strawberry allergy symptoms could be reduced by appropriate selection of cultivars, cultivating methods, and cooking methods.

Research Products

• [Journal Article] Expression of strawberry allergen Fra a 1 gene during fruit ripening (2014)
  • Author(s): Futsuki, D., Nitta, Y., Iduhara, M., Tsuruta, H., Tsugehara, T. and Uno, Y.
  • Journal Title: Acta Horticulturae Volume: (in press)
  • Peer Reviewed
• [Journal Article] Expression of strawberry allergen Fra a 1 gene during fruit ripening. (2014)
  • Author(s): Futsuki, D., Nitta, Y., Iduhara, M., Tsuruta, H., Tsugehara, T. and Uno, Y.
  • Journal Title: Acta Horticulturae Volume: in press
  • Peer Reviewed
• [Journal Article] Analysis of IgE binding capacity and stress inducibility of strawberry allergen Fra a 1 (2013)
  • Author(s): Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M. and Uno, Y.
  • Journal Title: International Strawberry Congress, Antwerp Volume: (USB) Pages: 6-6
• [Journal Article] Comparison of transcript and protein levels of strawberry allergen Fra a 1 among different cultivars (2013)
  • Author(s): Narukami, M., Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M., Noguchi, Y. and Uno, Y.
  • Journal Title: International Strawberry Congress, Antwerp Volume: (USB) Pages: 6-6
• [Journal Article] Comparison of transcript and protein levels of strawberry allergen Fra a 1 among different cultivars. (2013)
  • Author(s): Narukami, M., Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M., Noguchi, Y. and Uno, Y.
  • Journal Title: International Strawberry Congress (Proceedings) Volume: USB
• [Journal Article] Analysis of IgE binding capacity and stress inducibility of strawberry allergen Fra a 1. (2013)
  • Author(s): Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M. and Uno, Y.
  • Journal Title: International Strawberry Congress (Proceedings) Volume: USB
• [Journal Article] Varietal Differences in Transcript and Protein Levels of Strawberry Allergen Fra a 1 (2013)
  • Author(s): Narukami, M., Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M., Noguchi, Y. and Uno, Y.
  • Journal Title: HortScience Volume: 48
• [Journal Article] Comparison of IgE Binding Capacity and Expression Analysis of Strawberry Allergen Fra a 1 (2013)
  • Author(s): Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M. and Uno, Y.
  • Journal Title: HortScience Volume: 48
• [Journal Article] The Temperature Dependence of Fra a 1 Protein Structure (2013)
  • Author(s): Yoko Nitta, Daisuke Futsuki, Takeshi Nabe, Hiroki Tsuruta, Miho Iduhara,Yuichi Uno, Koichi Matsuo
  • Journal Title: HiSOR Activity Report 2012 Volume: 未定
• [Journal Article] The temperature dependence of Fra a 1 protein structure (2012)
  • Author(s): Nitta, Y., Futsuki, D., Nabe, T., Tsuruta, H., Iduhara, M., Uno, Y. and Matsuo, K.
  • Journal Title: HiSOR Activity Report 2012 Pages: 132-133
• [Presentation] イチゴアレルゲンFra a 1のIgE反応性と果実における局在 (2013)
  • Author(s): 夫津木大輔,奈邉健,新田陽子,鶴田宏樹,厳原美穂,野口裕司,宇野雄一
  • Organizer: 園芸学会平成25年度秋季大会
  • Place of Presentation: 岩手大学,園芸学研究
• [Presentation] Analysis of IgE binding capacity and stress inducibility of strawberry allergen Fra a 1, Book of abstracts (2013)
  • Author(s): Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M. and Uno, Y.
  • Organizer: International Strawberry Congress
  • Place of Presentation: Antwerp (Belgium)
• [Presentation] Comparison of transcript and protein levels of strawberry allergen Fra a 1 among different cultivars,V (2013)
  • Author(s): Narukami, M., Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M., Noguchi, Y. and Uno, Y.
  • Organizer: International Strawberry Congress
  • Place of Presentation: Antwerp (Belgium)
• [Presentation] Comparison of IgE binding capacity and expression analysis of strawberry allergen Fra a 1 (2013)
  • Author(s): Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M. and Uno, Y.
  • Organizer: ASHS (American Society for Horticultural Science) Annual Congress
  • Place of Presentation: Palm Desert (California, USA)
• [Presentation] Varietal differences in transcript and protein levels of strawberry allergen Fra a 1 (2013)
  • Author(s): Narukami, M., Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M., Noguchi, Y. and Uno, Y.
  • Organizer: ASHS (American Society for Horticultural Science) Annual Congress
  • Place of Presentation: Palm Desert (California, USA)
• [Presentation] Comparison of transcript and protein levels of strawberry allergen Fra a 1 among different cultivars. (2013)
  • Author(s): Narukami, M., Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M., Noguchi, Y. and Uno, Y.
  • Organizer: International Strawberry Congress 2013
  • Place of Presentation: Antwerp (Belgium)
• [Presentation] Analysis of IgE binding capacity and stress inducibility of strawberry allergen Fra a 1. (2013)
  • Author(s): Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M. and Uno, Y. (2013)
  • Organizer: International Strawberry Congress 2013
  • Place of Presentation: Antwerp (Belgium)
• [Presentation] Varietal Differences in Transcript and Protein Levels of Strawberry Allergen Fra a 1. (2013)
  • Author(s): Narukami, M., Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M., Noguchi, Y. and Uno, Y.
  • Organizer: American Society for Horticultural Science Annual Congress 2013
  • Place of Presentation: Palm Desert (California, USA)
• [Presentation] Comparison of IgE Binding Capacity and Expression Analysis of Strawberry Allergen Fra a 1. (2013)
  • Author(s): Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M. and Uno, Y. (2013)
  • Organizer: American Society for Horticultural Science Annual Congress 2013
  • Place of Presentation: Palm Desert (California, USA)
• [Presentation] イチゴアレルゲンFra a 1のIgE反応性と果実における局在 (2013)
  • Author(s): 夫津木大輔、奈邉健、新田陽子、鶴田宏樹、厳原美穂、野口裕司、宇野雄一
  • Organizer: 園芸学会平成25年度秋季大会
  • Place of Presentation: 岩手大学
• [Presentation] Comparison of IgE Binding Capacity and Expression Analysis of Strawberry Allergen Fra a 1 (2013)
  • Author(s): Daisuke Futsuki, Takeshi Nabe, Yoko Nitta, Hiroki Tsuruta, Kiyoshi Yamazaki, Miho Iduhara and Yuichi Uno
  • Organizer: 2013 American Society for Horticultural Science Annual Conference
  • Place of Presentation: Palm Desert, California
• [Presentation] Varietal Differences in Transcript and Protein Levels of Strawberry Allergen Fra a1 (2013)
  • Author(s): Manabu Narukami, Daisuke Futsuki, Takeshi Nabe, Yoko Nitta, Hiroki Tsuruta, Kiyoshi Yamazaki, Miho Iduhara, Yuji Noguchi and Yuichi Uno
  • Organizer: 2013 American Society for Horticultural Science Annual Conference
  • Place of Presentation: Palm Desert, California
• [Presentation] Analysis of IgE binding capacity and stress inducibility of strawberry allergen Fra a 1 (2013)
  • Author(s): Daisuke Futsuki, Takeshi Nabe, Yoko Nitta, Hiroki Tsuruta, Kiyoshi Yamazaki, Miho Iduhara and Yuichi Uno
  • Organizer: International Strawberry Congress 2013
  • Place of Presentation: Antwerp, Belgium
• [Presentation] Comparison of transcript and protein levels of strawberry allergen Fra a 1 among different cultivars (2013)
  • Author(s): Manabu Narukami, Daisuke Futsuki, Takeshi Nabe, Yoko Nitta, Hiroki Tsuruta, Kiyoshi Yamazaki, Miho Iduhara, Yuji Noguchi and Yuichi Uno
  • Organizer: International Strawberry Congress 2013
  • Place of Presentation: Antwerp, Belgium
• [Presentation] イチゴアレルゲンをコードするFra a 1遺伝子の解析 (2012)
  • Author(s): 夫津木大輔,奈邉健,新田陽子,鶴田宏樹,山崎清志,厳原美穂,宇野雄一
  • Organizer: 神戸大学研究基盤センター若手フロンティア研究会
  • Place of Presentation: 神戸大学
  • Year and Date: 2012-12-25
• [Presentation] イチゴアレルゲンをコードするFra a 1遺伝子の単離と発現解析 (2012)
  • Author(s): 夫津木大輔,奈邉健,新田陽子,鶴田宏樹,厳原美穂,宇野雄一
  • Organizer: 園芸学会平成24年度秋季大会
  • Place of Presentation: 福井県立大学,園芸学研究
• [Presentation] イチゴアレルゲンをコードするFra a 1遺伝子の単離と発現解析 (2012)
  • Author(s): 夫津木大輔、奈邉健、新田陽子、鶴田宏樹、厳原美穂、宇野雄一
  • Organizer: 園芸学会平成24年度秋季大会
  • Place of Presentation: 福井
• [Presentation] イチゴアレルゲンをコードするFra a 1遺伝子の解析 (2012)
  • Author(s): 夫津木大輔、奈邉健、新田陽子、鶴田宏樹、山崎清志、厳原美穂、宇野雄一
  • Organizer: 神戸大学研究基盤センター若手フロンティア研究会2012
  • Place of Presentation: 神戸