| Project/Area Number |
24658032
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TOMINAGA Rumi 広島大学, 生物圏科学研究科, 講師 (20373334)
SAWA Shinichiro 熊本大学, 自然科学研究科, 教授 (00315748)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 園芸学 / メリステム / ペプチドホルモン / 果樹 / 組織培養 / 根 |
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| Outline of Final Research Achievements |
CLE25, whose activity is higher than CLV3, a plant peptide hormone regulating development of the shoot apical meristem, was used in tissue culture of fruit trees. The roots from Japanese persimmon shoots growing in the medium supplemented with CLE25 were found to become thin, and the microscopic observation on the roots revealed that the number of cells in cortical layer decreased. However, the roots from grape shoots treated with CLE25 did not become thin, although their growth was inhibited by CLE25. We identified five CLV3-like genes from grape. Expression analyses showed that the five grape CLV3-like genes are expressed in leaves, stems, roots and axillary buds with significant differences in their levels of expression. The differential expression suggests that they play different roles in different organs and developmental stages.
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