Study of genes involved in acylquinic acid biosynthesis toward development of novel method for flower color modification

• Project/Area Number: 24658035
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticulture/Landscape architecture
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: NODA NAONOBU 独立行政法人農業・食品産業技術総合研究機構, 花き研究所花き研究領域, 主任研究員 (10455313)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 花色 / コピグメント / アシル化キナ酸 / アントシアニン / フラボノイド

Outline of Final Research Achievements

In this study, to regurate co-pigmentation of anthocyanin and co-pigment in the flower, acylquinic acid biosynthetic genes were isolated and characterized. Acylquinic acids, flavonoids and anthocyanins responsible for the flower coloration of chicory, artichoke, hydrangea, and chrysanthemum were ientified. The cDNA homologs encoding hydroxycinnamoyl-CoA: shikimate/quinate hydroxycinnamoyltransferase (HCT) and hydroxycinnamoyl-CoA: quinate hydroxycinnamoyltransferase (HQT) were cloned from artichoke, chrysanthemum and hydrangea.

Research Products

• [Journal Article] Structural basis for acceptor-substrate recognition of UDP-glucose: Anthocyanidin 3-O-glucosyltransferase from Clitoria ternatea (2015)
  • Author(s): Hiromoto T, Honjo E, Noda N, Tamada T, Kazuma K, Suzuki M, Blaber M, Kuroki R
  • Journal Title: Protein Science Volume: 24 Issue: 3 Pages: 395-407
  • DOI: 10.1002/pro.2630
  • Peer Reviewed
• [Presentation] アジサイの赤色覆輪品種の色素構成と遺伝特性 (2015)
  • Author(s): 小玉雅晴，田邊雄太，野田尚信，中山真義
  • Organizer: 園芸学会
  • Place of Presentation: 千葉大学（西千葉キャンパス）
  • Year and Date: 2015-03-28 – 2015-03-29
• [Presentation] 青色あるいは赤色を安定に発色するアジサイ品種の色素関連成分の特徴 (2014)
  • Author(s): 小玉雅晴，田邊雄太，野田尚信，中山真義
  • Organizer: 園芸学会
  • Place of Presentation: 佐賀大学（本庄キャンパス）
  • Year and Date: 2014-09-27 – 2014-09-29
• [Presentation] Metabolic engineering for methylated anthocyanins production in chrysanthemums (2014)
  • Author(s): Noda N, Nakamura N, Yoshioka S, Kishimoto S, Tanaka Y, Aida R
  • Organizer: XXVIIth International Conference on Polyphenols & 8th Tannin Conference
  • Place of Presentation: 名古屋大学（東山キャンパス）
  • Year and Date: 2014-09-02 – 2014-09-06