| Project/Area Number |
24658036
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant pathology
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| Research Institution | Hirosaki University |
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Principal Investigator |
SANO Teruo 弘前大学, 農学生命科学部, 教授 (30142699)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | サブウイルスRNA病原体 / ウイロイド / サテライトRNA / リボザイム / ヘアピン型リボザイム / ハンマーヘッド型リボザイム / 環状1本鎖RNA / サブウイルスＲＮＡ病原体 / サテライトＲＮＡ |
|---|
| Research Abstract |
Subviral RNA pathogen is a group of small RNA consisting of viroid which replicates autonomously depending on host transcription machinery and virus satellite RNA which replicates depending on host virus. Among them, viroid and viroid-like small circular satellite RNA are thought to be a same origin because they share similar molecular size, structure, and functions such as ribozyme activity.
Here in this research, we have analyzed a new viroid-like small circular RNA isolated from mulberry (Morus alba) cultivated in China on the molecular structures, genetic diversity and new ribozyme activity found in the minus strand, and detected a couple of mutations which could change the potential of ribozyme activity. Taking the lacking of ability to replicate autonomously and the structural similarity of new ribozyme to hairpin-ribozyme in considerations, Mulberry small circular RNA can be placed in small circular viral satellite RNA group.
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