| Project/Area Number |
24658038
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant pathology
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| Research Institution | Utsunomiya University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | キュウリモザイクウイルス / サテライトＲＮＡ / 出現 / 変異 / サテライトRNA / 感染性クローン / 接種 / 継代 / cucumber mosaic virus / satellite RNA |
|---|
| Research Abstract |
Cucumber mosaic virus (CMV) contains satellite RNA (satRNA), however,
The origin of satRNA is remains unexplained. The infectious clone of CMV-Pepo was inoculated on Nicotiana benthamiana and then the progeny virus was repeatedly passaged to new plants under insect-free greenhouse conditions. A satRNA emerged after repeated sap-inoculation of CMV-Pepo, but the emerging condition of satRNA has been unclear. The sequences of the emerged satRNA and its progeny molecules were determined and compared, indicating that the sequence of satRNA is unstable. Since the sequence of CMV satRNAs contain siRNAs of CMV-RNA and miRNAs of plant mRNA, the re-editing of siRNA /miRNA might be related to CMV satRNAs. Further research is necessarily to clear the nature of the origin of CMV satRNAs.
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