• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Building blocks method, efficient gene silencing of multiple genes

Research Project

Project/Area Number 24658040
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Plant pathology
Research InstitutionKobe University

Principal Investigator

NAKAYASHIKI Hitoshi  神戸大学, (連合)農学研究科(研究院), 教授 (50252804)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsいもち病菌 / 細胞壁分解酵素 / RNAi / 遺伝子サイレンシング / カスパーゼ / 細胞壁加水分解酵素
Research Abstract

In the post-genomics era, a major obstacle to functional genomics is functional redundancy among gene family members. To overcome this difficulty, we have developed an RNAi strategy designated building blocks method, in which a 40-60 bp sequence is chosen from a target gene, and such sequences from multiple gene family members were combined to make an artificial RNAi trigger by synthetic DNA. In this study, the building blocks method was applied to knock-down (KD) two gene families, xylanases and cellulases, to elucidate their roles in the pathogenicity of the blast fungus, Magnaporthe oryzae. In the resulting KD mutants, the transcript levels of all the target genes were significantly decreased. The number of lesions, rate of penetration, and extent of infected cells were all reduced in KD mutant-infected leaves. Thus, we concluded that xylanases and cellulases play significant roles in vertical penetration and horizontal expansion of M. oryzae in infected plants.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2013 2012 Other

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (3 results) Remarks (2 results)

  • [Journal Article] Substrate-induced transcriptional activation of the MoCel7C cellulase gene is associated with methylation of histone H3 at lysine 4 in the rice blast fungus Magnaporthe oryzae2013

    • Author(s)
      Vu BV, Pham KT, Nakayashiki H
    • Journal Title

      Appl Environ Microbiol

      Volume: 79 Pages: 6823-6832

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Appressorium-localized NADPH oxidase B is essential for aggressiveness and pathogenicity in the host-specific, toxin-producing fungus Alternaria alternata Japanese pear pathotype2013

    • Author(s)
      Morita Y, Hyon GS, Hosogi N, Miyata N, Nakayashiki H, Muranaka Y, Inada N, Park P, Ikeda K
    • Journal Title

      Mol Plant Pathol

      Volume: 14 Pages: 365-378

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning of the apoptosis-related calcium-binding protein AsALG-2 in Avena sativa2013

    • Author(s)
      Hoat TX, Nakayashiki H, Yang Q, Tosa Y, Mayama S
    • Journal Title

      Mol Plant Pathol

      Volume: 14 Pages: 222-229

    • Related Report
      2013 Final Research Report
  • [Journal Article] Substrate-induced transcriptional activation of the MoCel7C cellulase gene is associated with methylation of histone H3 at lysine 4 in the rice blast fungus Magnaporthe oryzae.2013

    • Author(s)
      Vu BV, Pham KT, Nakayashiki H.
    • Journal Title

      Appl Environ Microbiol

      Volume: 79 Issue: 21 Pages: 6823-6832

    • DOI

      10.1128/aem.02082-13

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Appressorium-localized NADPH oxidase B is essential for aggressiveness and pathogenicity in the host-specific, toxin-producing fungus Alternaria alternata Japanese pear pathotype.2013

    • Author(s)
      Morita Y
    • Journal Title

      Mol Plant Pathol

      Volume: 14. Issue: 4 Pages: 365-378

    • DOI

      10.1111/mpp.12013

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cellulases belonging to glycoside hydrolase families 6 and 7 contribute to the virulence of Magnaporthe oryzae2012

    • Author(s)
      Vu, V.B., Itoh, K., Nguyen, Q.B. and Nakayashiki, H
    • Journal Title

      Molecular Plant-Microbe Interaction

      Volume: 25 Pages: 1135-1141

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cellulases belonging to glycoside hydrolase families 6 and 7 contribute to the virulence of Magnaporthe oryzae.2012

    • Author(s)
      Van Vu Ba
    • Journal Title

      Mol Plant Microbe Interact

      Volume: 25 Issue: 9 Pages: 1135-1141

    • DOI

      10.1094/mpmi-02-12-0043-r

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning of the apoptosis-related calcium-binding protein AsALG-2 in Avena sativa.2012

    • Author(s)
      Hoat T.X.
    • Journal Title

      Mol Plant Pathol

      Volume: 14 Issue: 3 Pages: 222-229

    • DOI

      10.1111/j.1364-3703.2012.00844.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] いもち病菌における新たな遺伝子解析手法、Building Blocks法の検討2012

    • Author(s)
      村部知里、河南浩美、伊藤賢司、中屋敷均
    • Organizer
      平成24年度日本植物病理学会関西部会
    • Place of Presentation
      鳥取市鳥銀文化会館
    • Year and Date
      2012-09-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Histone lysine methyltransferases in Magnaporthe oryzae are involved in various aspects of infection and pathogenesis2012

    • Author(s)
      Pham, K. T. M., Vu, B. V., Nguyen, Q. B., Ikeda, K., and Nakayashiki, H.
    • Organizer
      10th International Congress of Plant Pathology
    • Place of Presentation
      China
    • Year and Date
      2012-08-29
    • Related Report
      2013 Final Research Report
  • [Presentation] いもち病菌における新たな遺伝子解析手法Building Blocks法の検討2012

    • Author(s)
      村部ら
    • Organizer
      日本植物病理学会関西部会
    • Place of Presentation
      鳥取
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.ans.kobe-u.ac.jp/jyukensei/e-bio/guide/structure.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 細胞機能構造学

    • URL

      http://www.ans.kobe-u.ac.jp/jyukensei/e-bio/guide/structure.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi