Development of novel method for searching plant disease resistance gene based on the pathogen perception mechanism

• Project/Area Number: 24658044
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Ehime University
• Principal Investigator: KOBAYASHI Kappei 愛媛大学, 農学部, 准教授 (40244587)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 植物ウイルス / 抵抗性 / タンパク質間相互作用 / 植物 / ウイルス / 抵抗性遺伝子 / 酵母ツーハイブリッド法

Research Abstract

We tested whether or not it is possible to isolate novel plant disease resistance gene based on the interaction with protein factors known to interact with known resistance protein. Partial cDNA sequences homologous to pepper tobamovirus resistance gene, L, from an eggplant cultivar were screened for the interaction with LIPM1 and LIPM2, both of which were identified as interaction partners of pepper L resistance protein. The results suggest that the both LIPM1 and LIPM2 interact with diverse L-homolog sequences from eggplant and that they are unlikely to be involved in the determination of pathogen specificity of resistance protein. Gene-silencing study suggest that LIPM2 has a role in the regulation of cell-death signaling.

Research Products

• [Journal Article] Breakdown of plant virus resistance : Can we predict and extend the durability of virus resistance? (2014)
  • Author(s): Kobayashi, K., Sekine, K.-T. and Nishiguchi, M.
  • Journal Title: Journal of General Plant Pathology Volume: 80(印刷中)
  • NAID: 130004779388
  • URL: http://link.springer.com/article/10.1007/s10327-014-0527-1
  • Peer Reviewed
• [Journal Article] Identification and characterization of Lisianthus necrotic ringspot virus, a novel distinct tospovirus species causing necrotic disease of lisianthus (Eustoma grandiflorum) (2014)
  • Author(s): Shimomoto, Y., Kobayashi, K. and Okuda, M.
  • Journal Title: Journal of General Plant Pathology Volume: 80 Issue: 2 Pages: 169-175
  • DOI: 10.1007/s10327-014-0503-9
  • Peer Reviewed
• [Journal Article] Establishment of an agroinoculation system for broad bean wilt virus 2 (2013)
  • Author(s): Atsumi, G., Tomita, R., Kobayashi, K. and Sekine, K.-T.
  • Journal Title: Archives of Virology Volume: 158 Issue: 7 Pages: 1549-1554
  • DOI: 10.1007/s00705-013-1625-7
  • Peer Reviewed
• [Journal Article] Prevalence and genetic diversity of an unusual virus associated with Kobo-sho disease of gentian in Japan (2013)
  • Author(s): Atsumi, G., Tomita, R., Kobayashi, K. and Sekine, K.-T.
  • Journal Title: Journal of General Virology Volume: 94 Issue: 10 Pages: 2360-2365
  • DOI: 10.1099/vir.0.053637-0
  • Peer Reviewed
• [Journal Article] Identification of the domains of Cauliflower mosaic virus protein P6 responsible for suppression of RNA-silencing and salicylic acid-signaling (2013)
  • Author(s): Laird, J., McInally, C., Carr, C., Doddiah, S., Yates, G., Chrysanthou, E., Khattab, A., Love, A. J., Geri, C., Sadanandom, A., Smith, B. O., Kobayashi, K. and Milner, J.
  • Journal Title: Journal of General Virology Volume: 94 Issue: 12 Pages: 2777-2789
  • DOI: 10.1099/vir.0.057729-0
  • Peer Reviewed
• [Journal Article] Gentian Kobu-sho-associated virus: a tentative, novel double-stranded RNA virus that is relevant to gentian Kobu-sho syndrome (2013)
  • Author(s): 小林括平
  • Journal Title: Journal of General Plant Pathology Volume: 79 Issue: 1 Pages: 56-63
  • DOI: 10.1007/s10327-012-0423-5
  • NAID: 10031145676
  • Peer Reviewed
• [Journal Article] Functional differentiation in the LRR domains of closely related plant virus resistance proteins that recognize common Avr proteins (2012)
  • Author(s): Sekine, K.-T., Tomita, R., Takeuchi, S., Atsumi, G., Saitoh, H., Mizumoto, H., Kiba, A., Yamaoka, N.,Nishiguchi, M., Hikichi, Y. and Kobayashi, K.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 25 Pages: 1219-1229
  • Peer Reviewed
• [Journal Article] Functional Differentiation in the Leucine-Rich Repeat Domains of Closely Related Plant Virus-Resistance Proteins That Recognize Common Avr Proteins (2012)
  • Author(s): 関根健太郎
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 25 Issue: 9 Pages: 1219-1229
  • DOI: 10.1094/mpmi-11-11-0289
  • Peer Reviewed
• [Presentation] トウガラシ微斑ウイルス外被タンパク質変異株におけるN´抵抗性を回避するために必須な変異の同定 (2013)
  • Author(s): 出原健吾・関根健太郎・八丈野孝・山岡直人・西口正通・小林括平
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 岡山大学
• [Presentation] Further investigation on graft-transmission of RNA silencing and tobamovirus resistance to non-transgenic scions of tobacco and tomato. (2013)
  • Author(s): Ali, M. E., Kobayashi, K., Yaeno, T., Yamaoka, N. and Nishiguchi, M.
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 岡山大学
• [Presentation] Characterization of Rice Heme Activator Protein gGene (OsHAP2E) in response to fungal and bacterial infections. (2013)
  • Author(s): Alam, M. M., Nakamura, N., Ichikawa, H., Kobayashi, K., Yaeno, T., Yamaoka, N. and Nishiguchi, M.
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 岡山大学
• [Presentation] オオムギうどんこ病菌（Blumeria graminis）の感染過程における第一発芽管の役割 (2013)
  • Author(s): 菅井維之・新崎裕樹・八丈野孝・小林括平・西口正通・山岡直人
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 岡山大学
• [Presentation] オオムギうどんこ病菌エフェクターの分泌及び宿主細胞侵入機構の解析 (2013)
  • Author(s): 香口智宏・菅井維之・中下香・久野裕・小林括平・西口正通・山岡直人・八丈野孝
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 岡山大学
• [Presentation] Expression of Cauliflower mosaic virus Transactivator/viroplasmin in transgenic tobacco under the control of dexamethasone-inducible promoter (2013)
  • Author(s): Waliullah Sumyya
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 岐阜大学（岐阜県岐阜市）
• [Presentation] Avr機能獲得変異体を用いたトバモウイルス抵抗性タンパク質による認識機構の解析 (2013)
  • Author(s): 関根健太郎
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 岐阜大学（岐阜県岐阜市）
• [Presentation] リンドウこぶ症関連ウイルスが持つと考えられるこぶ症誘導因子の探索 (2013)
  • Author(s): 厚見剛
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 岐阜大学（岐阜県岐阜市）
• [Presentation] The binding affinity to viral coat proteins determines the recognition specificity of allelic L tobamovirus resistance proteins (2012)
  • Author(s): Sekine K.-T., Tomita R., Atsumi G., Chen H., Kaido M., Yamaoka N., Nishiguchi M., Okuno T. and Kobayashi K.
  • Organizer: International congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都
• [Presentation] Evaluation of the durability of N´ resistance gene to Pepper mild mottle virus using random mutagenesis of coat protein genes (2012)
  • Author(s): 出原健吾
  • Organizer: IS-MPMI 2012 XV International Congress
  • Place of Presentation: 京都国際会館（京都府京都市）
• [Presentation] The binding affinity to viral coat proteins determines the recognition specificity of allelic L tobamovirus resistance proteins (2012)
  • Author(s): 関根健太郎
  • Organizer: IS-MPMI 2012 XV International Congress
  • Place of Presentation: 京都国際会館（京都府京都市）
• [Presentation] Expression of viral partial sequence induces unique developmental abnormalities in stem and leaf tissues (2012)
  • Author(s): 厚見剛
  • Organizer: IS-MPMI 2012 XV International Congress
  • Place of Presentation: 京都国際会館（京都府京都市）
• [Presentation] N´遺伝子抵抗性を打破したトウガラシ微斑ウイルス外被タンパク質人工突然変異株の特徴付け (2012)
  • Author(s): 出原健吾
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: とりぎん文化会館（鳥取県鳥取市）