Development of novel method for searching plant disease resistance gene based on the pathogen perception mechanism
| Project/Area Number |
24658044
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant pathology
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 植物ウイルス / 抵抗性 / タンパク質間相互作用 / 植物 / ウイルス / 抵抗性遺伝子 / 酵母ツーハイブリッド法 |
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| Research Abstract |
We tested whether or not it is possible to isolate novel plant disease resistance gene based on the interaction with protein factors known to interact with known resistance protein. Partial cDNA sequences homologous to pepper tobamovirus resistance gene, L, from an eggplant cultivar were screened for the interaction with LIPM1 and LIPM2, both of which were identified as interaction partners of pepper L resistance protein. The results suggest that the both LIPM1 and LIPM2 interact with diverse L-homolog sequences from eggplant and that they are unlikely to be involved in the determination of pathogen specificity of resistance protein. Gene-silencing study suggest that LIPM2 has a role in the regulation of cell-death signaling.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Identification of the domains of Cauliflower mosaic virus protein P6 responsible for suppression of RNA-silencing and salicylic acid-signaling2013
Author(s)
Laird, J., McInally, C., Carr, C., Doddiah, S., Yates, G., Chrysanthou, E., Khattab, A., Love, A. J., Geri, C., Sadanandom, A., Smith, B. O., Kobayashi, K. and Milner, J.
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Journal Title
Journal of General Virology
Volume: 94
Issue: 12
Pages: 2777-2789
DOI
Related Report
Peer Reviewed
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[Journal Article] Functional differentiation in the LRR domains of closely related plant virus resistance proteins that recognize common Avr proteins2012
Author(s)
Sekine, K.-T., Tomita, R., Takeuchi, S., Atsumi, G., Saitoh, H., Mizumoto, H., Kiba, A., Yamaoka, N.,Nishiguchi, M., Hikichi, Y. and Kobayashi, K.
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Journal Title
Molecular Plant-Microbe Interactions
Volume: 25
Pages: 1219-1229
Related Report
Peer Reviewed
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[Presentation] Characterization of Rice Heme Activator Protein gGene (OsHAP2E) in response to fungal and bacterial infections.2013
Author(s)
Alam, M. M., Nakamura, N., Ichikawa, H., Kobayashi, K., Yaeno, T., Yamaoka, N. and Nishiguchi, M.
Organizer
日本植物病理学会関西部会
Place of Presentation
岡山大学
Related Report
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