Mapping of mutation point on holokinetic chromosome in Bombyx mori

• Project/Area Number: 24658046
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: Iwate University
• Principal Investigator: SAHARA Ken 岩手大学, 農学部, 教授 (30241368)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: BAC-FISH / 染色体突然変異 / 相互転座 / 付着 / カイコゲノム / カイコ / 染色体 / 突然変異系統 / 染色体付着 / 染色体転座 / NBRP / BAC / FISH

Research Abstract

I have analyzed chromosome mutation in 3 Bombyx mori strains (r501, r52 and r53) provided through national bioresource project (NBRP). BAC-FISH (fluorescence in situ hybridization using bacterial artificial chromosomes) revealed the exact direction of the fusion between chromosomes 23 and 25. I defined reciprocal translocation is the cause for the mutation in r52 and r53 strains. The double strand breakage and further fusion for each mutation chromosomes were also analyzed by the BAC-FISH. These detected a bridge BAC responsible for a mutation chromosome. I am trying to reveal the mutation point by PCR as a reference of Bombyx mori genome databes.

Research Products

• [Journal Article] BAC-FISHを用いたカイコ染色体突然変異系統の解析 (2013)
  • Author(s): 鈴木一生・安河内祐二・伴野豊・佐原健
  • Journal Title: 東北蚕糸・昆虫利用研究 Volume: 38 Pages: 4-7
• [Presentation] カイコ染色体突然変異系統の細胞遺伝学的解析 (2014)
  • Author(s): 鈴木一生・安河内祐二・伴野豊・佐原健
  • Organizer: 日本蚕糸学会第84回大会日本大学生物資源科学部
  • Place of Presentation: 藤沢市
• [Presentation] カイコ染色体突然変異系統の細胞遺伝学的解析 (2014)
  • Author(s): 鈴木一生・安河内祐二・伴野豊・佐原健
  • Organizer: 日本蚕糸学会第84回大会
  • Place of Presentation: 神奈川県藤沢市亀井野1866 日本大学生物資源科学部
• [Presentation] BAC-FISHによるカイコ染色体突然変異系統の解析 (2013)
  • Author(s): 鈴木一生・伴野豊・佐原健
  • Organizer: 日本昆虫学会東北支部第60回大会
  • Place of Presentation: 山形市(蔵王温泉ホテル樹林)
  • Year and Date: 2013-07-27
• [Presentation] BAC-FISHによるカイコ染色体突然変異系統の解析 (2013)
  • Author(s): 鈴木一生・伴野豊・佐原健
  • Organizer: 日本昆虫学会東北支部第60回大会
  • Place of Presentation: 山形市蔵王温泉814 ホテル樹林
• [Presentation] 鱗翅目昆虫における染色体同定 (2012)
  • Author(s): 佐原健・吉戸敦生・安河内祐二
  • Organizer: 日本昆虫学会東北支部第59回大会
  • Place of Presentation: 八幡平市(八幡平ハイツ)
  • Year and Date: 2012-08-04