Development of odorant sensor silkmoths by genetic modification of sex pheromone detection system
| Project/Area Number |
24658049
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied entomology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAKURAI Takeshi 東京大学, 先端科学技術研究センター, 特任助教 (20506761)
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| Co-Investigator(Kenkyū-buntansha) |
UCHINO Keiro 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 研究員 (90563627)
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| Co-Investigator(Renkei-kenkyūsha) |
MITSUNO Hidefumi 東京大学, 先端科学技術研究センター, 特任研究員 (60511855)
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| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 昆虫 / 生体機能利用 / 匂いセンサ / カイコガ / 嗅覚受容体 |
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| Research Abstract |
We generated transgenic silkmoths expressing an odorant receptor for general odorants (Or82a) from the fruit fly under the control of the sex pheromone receptor gene promoter in the silkmoth. Male moths expressing Or82a display pheromone searching behavior in response to geranyl acetate, one of ligands of Or82a, in dose-dependent manner. Expression of Or82a does not affect projection pattern of bombykol receptor neurons. These results indicate that odor selectivity for initiation of pheromone searching behavior can be genetically modified by ectopic expression of general odorant receptors in bombykol receptor neurons, and open the way to utilize male silkmoths as biosensors for various odorants.
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Report
(2 results)
Research Products
(10 results)
