The mechanism by which parasitoid wasp Asobara japaonoca

• Project/Area Number: 24658053
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: Saga University
• Principal Investigator: HAYAKAWA Yoichi 佐賀大学, 農学部, 教授 (50164926)
• Research Collaborator: FURIHATA Shunsuke
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 寄生蜂 / 毒液 / 細胞性免疫 / 体液性免疫 / セリンプロテアーゼ / 宿主昆虫 / ショウジョウバエ / 殺虫成分 / 側輸卵管成分 / 解毒成分 / 自然免疫 / 細胞生免疫

Research Abstract

Endoparasitoid wasp Asobara japonica utilizes Drosophila melanogaster and the other broadly related Drosophila species as habitual hosts by using a notable feature of parasitism strategy. A. japonica females possess highly toxic venom and they inject the venom components with a lethal dose for the hosts at parasitism. A few seconds after the venom injection, they introduce eggs together with the lateral oviduct components that neutralize the detrimental effects of the venom toxin. In the present study, We demonstrated that the venom components impaired the host cellular immunity but did not affect the humoral immunity. Furthermore, injection of the venom components activated a serine protease-like enzyme activity approximately 100 times in the plasma 4 h after the injection. The neutralizing factor in the oviduct blocked the protease-like enzyme activation, while it was unable to neutralize the venom-induced detriment of the host hemocytes.

Research Products

• [Journal Article] Stimulation of Vitellogenin gene expression by permethrin in the brown planthopper, Nilaparvata lugens (Hemiptera : Auchenorrhyncha : Delphacidae) (2014)
  • Author(s): Khaeso,K., Matsumoto, H., Hayakawa, Y., and Tojo, S.
  • Journal Title: J. Pesticide Sci. Volume: (In press)
  • NAID: 130004725729
• [Journal Article] Changes of RNA virus infection rates and gut microbiota in young worker Apis mellifera (Hymenoptera : Apidae) of the chalkbrood-infected colony after a pollination task in a greenhouse (2014)
  • Author(s): Matsumoto, H., Nomura, S., Hayakawa, Y.
  • Journal Title: Applied Entomology and Zoology Volume: (in press)
  • NAID: 40020169409
• [Journal Article] Gain of long tonic immobility behavioral trait causes the red flour beetle to reduce anti-stress capacity (2014)
  • Author(s): Kiyotake, H., Matsumoto, H., Nakayama, S., Sakai, M., Miyatake, T., Ryuda, M., Hayakawa, Y.
  • Journal Title: J. Insect Physiol Volume: 60 Pages: 92-97
• [Journal Article] Immunoevasive protein (IEP)-containing surface layer covering polydnavirus particles is essential for viral infection (2014)
  • Author(s): Furihata, S., Tanaka, K., Ryuda, M., Ochiai, M., Matsumoto, H., Csikos, G. and Hayakawa, Y.
  • Journal Title: J. Invertebr. Pathol Volume: 115 Pages: 26-32
• [Journal Article] Immunoevasive protein (IEP)-containing surface layer covering polydnavirus particles is essential for viral infection. (2014)
  • Author(s): Furihata, S., Tanaka, K., Ryuda, M., Ochiai, M., Matsumoto, H., Csikos, G. and Hayakawa, Y.
  • Journal Title: J. Invertebr. Pathol. Volume: 115 Pages: 26-32
  • DOI: 10.1016/j.jip.2013.10.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gain of long tonic immobility behavioral trait causes the red flour beetle to reduce anti-stress capacity (2014)
  • Author(s): Kiyotake H, Matsumoto H, N akayama S, Sakai M, Miyata ke T, Ryuda M, and Hayakawa Y
  • Journal Title: Journal of Insect Physiology Volume: 60 Pages: 92-97
  • DOI: 10.1016/j.jinsphys.2013.11.008
  • Peer Reviewed
• [Journal Article] Changes of RNA virus infection rates and gut microbiota in young worker Apis mellifera (Hymenoptera: Apidae) of the chalkbrood-infected colony after a pollination task in a greenhouse. (2014)
  • Author(s): Matsumoto, H., Nomura, S., Hayakawa, Y.
  • Journal Title: Applied Entomology and Zoology Volume: 49 Issue: 3 Pages: 395-402
  • DOI: 10.1007/s13355-014-0261-3
  • NAID: 40020169409
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Stimulation of vitellogenin gene expression by permethrin in the brown planthopper, <i>Nilaparvata lugens</i> (Hemiptera: Auchenorrhyncha: Delphacidae) (2014)
  • Author(s): Khaeso, K., Matsumoto, H., Hayakawa, Y., Tojo, S.
  • Journal Title: Journal of Pesticide Science Volume: 39 Issue: 2 Pages: 98-104
  • DOI: 10.1584/jpestics.D13-052
  • NAID: 130004725729
  • ISSN: 1348-589X, 1349-0923
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Venom components of Asobara japonica impair cellular immune responses of host Drosophila melanogaster (2013)
  • Author(s): Furihata,S.X., Matsumoto, H., Kimura, M.T., Hayakawa, Y.
  • Journal Title: Archive of Insect Biochemistry and Physiology Volume: 83 Pages: 86-100
• [Journal Article] Activation of PLC by an endogenous cytokine (GBP) in Drosophila S3 cells and its application as a model for studying inositol phosphate signalling through ITPK1 (2012)
  • Author(s): Zhou, Y., Wu, S., Wang, H., Hayakawa, Y., Bird, G.S., and Shears, S.B.
  • Journal Title: Biochem. J Volume: 448 Pages: 273-283
• [Journal Article] Characteristics common to a cytokine family spanning five orders of insects (2012)
  • Author(s): Matsumoto, H., Tsuzuki, S., Date-Ito, A., Ohnishi, A., and Hayakawa, Y.
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 42 Pages: 446-454
• [Journal Article] Drosophila growth-blocking peptide-like factor mediates acute immune reactions during infectious and non-infectious stress (2012)
  • Author(s): Tsuzuki, S., Ochiai, M., Matsumoto, H., Kurata, S., Ohnishi A., Hayakawa, Y.
  • Journal Title: Sci. Rep. Volume: 2 Issue: 1 Pages: 210-210
  • DOI: 10.1038/srep00210
  • Peer Reviewed
• [Journal Article] A stress-responsive cytokine-like gene regulates insect larval growth (2012)
  • Author(s): Yamaguchi, K., Nomura, S., Matsumoto, H., Tsuzuki, S., Hayakawa, Y.
  • Journal Title: Insect Biochem.Molec.Biol. Volume: 42 Issue: 3 Pages: 183-192
  • DOI: 10.1016/j.ibmb.2011.11.009
  • Peer Reviewed
• [Journal Article] Characteristics common to a cytokine family spanning five orders of insects (2012)
  • Author(s): Matsumoto, H., Tsuzuki, S., Date-Ito, A., Ohnishi, A., Hayakawa, Y.
  • Journal Title: Insect Biochem.Molec.Biol. Volume: 42 Issue: 6 Pages: 446-454
  • DOI: 10.1016/j.ibmb.2012.03.001
  • Peer Reviewed
• [Journal Article] Activation of PLC by an endogenous cytokine (GBP) in Drosophila S3 cells and its application as a model for studying inositol phosphate signaling through ITPK1. (2012)
  • Author(s): Zhou, Y., Wu, S., Wang, H., Hayakawa, Y., Bird, G.S., and Shears, S.B.
  • Journal Title: Biochemical Journal Volume: 448 Issue: 2 Pages: 273-283
  • DOI: 10.1042/bj20120730
  • Peer Reviewed
• [Presentation] 宿主アワヨトウ幼虫からのカリヤコマユバチ脱出誘導因子の発見 (2014)
  • Author(s): 砂山貴志,内川雄貴,,落合正則,早川洋一
  • Organizer: 日本応用動物昆虫学会第57回大会
  • Place of Presentation: 高知大
  • Year and Date: 2014-03-28
• [Presentation] 宿主アワヨトウ幼虫からのカリヤコマユバチ脱出誘導因子の発見 (2014)
  • Author(s): 砂山貴志、内川雄貴、落合正則、早川洋一
  • Organizer: 応用動物昆虫学会
  • Place of Presentation: 高知
• [Presentation] フタホシコオロギ雄の性的不能期におけるTAGの5-HT量変化 (2013)
  • Author(s): 嬉正勝,早川洋一
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山大
  • Year and Date: 2013-09-28
• [Presentation] 寄生婆知asobara japonica毒液殺虫成分が宿主ショウジョウバエに及ぼす生理機能 (2013)
  • Author(s): 降幡駿介、早川洋一
  • Organizer: 第５７回日本応用動物昆虫学会大会
  • Place of Presentation: 日本大学生物資源科学科
• [Presentation] Biological functions of insect cytokine growth-blocking peptide (GBP) (2012)
  • Author(s): Yoichi Hayakawa
  • Organizer: International Congress of Entomology
  • Place of Presentation: Daegu, Korea
  • Invited
• [Remarks]
  • URL: http://extwww.cc.saga-u.ac.jp/~hayakayo/FRAME/index.html