| Project/Area Number |
24658053
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied entomology
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| Research Institution | Saga University |
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Principal Investigator |
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| Research Collaborator |
FURIHATA Shunsuke
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 寄生蜂 / 毒液 / 細胞性免疫 / 体液性免疫 / セリンプロテアーゼ / 宿主昆虫 / ショウジョウバエ / 殺虫成分 / 側輸卵管成分 / 解毒成分 / 自然免疫 / 細胞生免疫 |
|---|
| Research Abstract |
Endoparasitoid wasp Asobara japonica utilizes Drosophila melanogaster and the other broadly related Drosophila species as habitual hosts by using a notable feature of parasitism strategy. A. japonica females possess highly toxic venom and they inject the venom components with a lethal dose for the hosts at parasitism. A few seconds after the venom injection, they introduce eggs together with the lateral oviduct components that neutralize the detrimental effects of the venom toxin. In the present study, We demonstrated that the venom components impaired the host cellular immunity but did not affect the humoral immunity. Furthermore, injection of the venom components activated a serine protease-like enzyme activity approximately 100 times in the plasma 4 h after the injection. The neutralizing factor in the oviduct blocked the protease-like enzyme activation, while it was unable to neutralize the venom-induced detriment of the host hemocytes.
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