| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
When an Escherichia coli alanine auxotroph was grown at 37 degrees C in minimal medium containing varying concentrations of alanine (0.1-100 ug/ml) to the late log-phase, the number of suppressors that have no requirement for alanine decreased as the amount of alanine added in medium increased. These alanine-non-requiring suppressors appeared after 30 hours of incubation in minimal medium without alanine supplementation. In addition, individual suppressor clones isolated independently showed different levels of growth recovery in minimal medium. On the basis of these results, we could speculate the following scenario leading to appearance of suppressors: i) sensing of alanine deficiency, ii) metabolic change caused by an unknown signal transduction cascade, iii) introduction of random mutation(s) in the chromosome, and iv) selection of suppressors under the alanine-deficient conditions.
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