Analyses of molecular mechanism of stress-induced mutation toward control and prevention of the development of multiantibiotic resistance
Project/Area Number |
24658067
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
|
Research Institution | Tohoku University |
Principal Investigator |
YONEYAMA Hiroshi 東北大学, (連合)農学研究科(研究院), 准教授 (10220774)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 大腸菌 / アラニン要求変異株 / サプレッサ-変異株 / 突然変異 / アラニン飢餓ストレス / ストレス誘導性突然変異 / 多剤耐性 / サプレッサー変異 / アラニン飢餓 |
Outline of Final Research Achievements |
When an Escherichia coli alanine auxotroph was grown at 37 degrees C in minimal medium containing varying concentrations of alanine (0.1-100 ug/ml) to the late log-phase, the number of suppressors that have no requirement for alanine decreased as the amount of alanine added in medium increased. These alanine-non-requiring suppressors appeared after 30 hours of incubation in minimal medium without alanine supplementation. In addition, individual suppressor clones isolated independently showed different levels of growth recovery in minimal medium. On the basis of these results, we could speculate the following scenario leading to appearance of suppressors: i) sensing of alanine deficiency, ii) metabolic change caused by an unknown signal transduction cascade, iii) introduction of random mutation(s) in the chromosome, and iv) selection of suppressors under the alanine-deficient conditions.
|
Report
(4 results)
Research Products
(1 results)