| Project/Area Number |
24658069
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | scyllo-inositol / myo-inositol / Paracoccus sp. 43P / bioconversion / Paracoccus / inositol dehydrogenase |
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| Research Abstract |
In this study, we aimed to develop a scyllo-inositol (SI) production system from myo-inositol (MI), using MI dehydrogenase (IolG) and scyllo-inosose reductase (LgdA) of Paracoccus sp. 43P. In vitro reaction with both the enzymes produced 17.8 mM SI from 50 mM MI after 10 h reaction, showing that these enzymes can be used for SI production, with relatively high conversion rate (35%).
The iolE deletion mutant of strain 43P, which blocks downstream catabolism of scyllo-inosose, was constructed and used for bioconversion of MI to SI. This strain achieved about 20% conversion to SI after 96 h cultivation in a minimal medium with galactose as a carbon source.
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