Change of E. coli to an autotroph

• Project/Area Number: 24658071
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: ISHII Masaharu 東京大学, 農学生命科学研究科, 教授 (30193262)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 大腸菌 / 独立栄養 / x独立栄養 / ヒドロゲナーゼ / 還元的ペントースリン酸サイクル

Research Abstract

In order to change E. coli to an autotroph, heterologous expression of RubisCO (Ribulose bisphosphate carboxylase/ oxygenase), PRK (Phosphoribulokinase), and NAD reducing hydrogenase from Ralstonia eutropha were attempted. NAD reducing hydrogenase was successfully expressed under anaerobic growth condition. Also, expression of RubisCO and PRK has been done successfully. Now, final experiment for the checking of the change is being performed.