Establishment of Lysinibacillus collection and development of new microbial pesticides

• Project/Area Number: 24658073
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: OYAIZU Hiroshi 東京大学, 生物生産工学研究センター, 教授 (70177301)
• Research Collaborator: AKIMOTO Yuka
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: Ｌｙｓｉｎｉｂａｃｉｌｌｕｓ / 殺虫性結晶性タンパク質 / 菌株コレクション / Lysinibacillus / 結晶性タンパク質 / 殺虫性タンパク質

Outline of Final Research Achievements

In this study, microorganisms resistant to heat treatment at 90℃ were isolated from various kinds of dead insects collected in Japan. From the heat resistant bacteria Lysinibacillus-like bacteria which produce crystal proteins insecticidal to hemipteran were screened. Approximately five thousands heat resistant Bacillus-like bacteria were isolated from collected dead insects, and approximately five hundred Lysinibacillus-like bacteria were selected based on the 16S rRNA sequences. Characterization of crystal proteins were carried out by the sequencing of the amino acids. However, the various kinds of used primer sets did not work very well because of their genetic diversity.