| Project/Area Number |
24658075
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Mie University |
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Principal Investigator |
SAKKA Kazuo 三重大学, 生物資源学研究科, 教授 (20154031)
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| Co-Investigator(Kenkyū-buntansha) |
SAKKA Makiko 三重大学, 大学院生物資源学研究科, 学術研究員 (00422882)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 堆肥 / 好熱性細菌 / 好熱性酵素 / 微生物生態学 / 菌叢 / 細胞壁分解酵素 / 遺伝子資源 / リパーゼ / 古細菌 / キシラナーゼ |
|---|
| Research Abstract |
Temperature reached over 90 degrees centigrade in the upper position of the compost of the high-temperature composting system. Viable cell numbers by surface plate method was about 1/100 to 1/1000 of those by microscopic observation method, suggesting that unculturable bacteria were important in the high-temperature composting process. Bacillus species dominated in the early stage of composting, accounting for 36% of the total cell numbers, but Melghirimyces thermohalophilus accounted for 63%, indicating that bacterial flora drastically changed in the process of the composting. Genes encoding thermophilic enzymes were directly cloned from DNA isolated from the compost and thermophilic bacteria producing bacterial cell wall-degrading enzymes were isolated from the compost, indicating that the high-temperature compost was quite useful as the genetic resource for isolating thermophilic enzymes and bacteria.
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