| Project/Area Number |
24658076
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Setsunan University (2013)
Kyoto University (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | nitrogen fixation / biofertilizer / levan / phosphate concentration / G.diazotrophicus / endophytic bacteria / 非マメ科植物 / レバン / クオラムセンシング / 活性酸素種 / エンドファイト / 窒素固定細菌 / 窒素固定 / 酢酸菌 / Gluconacetobacter / リン酸 / 細胞外多糖 / ホモセリンラクトン |
|---|
| Research Abstract |
Due to the increasing cost of chemical nitrogenous fertilizer and concerns about contamination of soil and water, there is a need to reduce usage of chemical fertilizer. Plant-associated microorganisms containing nitrogenases have attracted attention as alternative biofertilizers, since nitrogenase catalyzes a reaction referred to as biological nitrogen fixation. Among endophytic nitrogen-fixing bacteria, we selected Gluconacetobacter diazotrophicus(Gdi) and analyzed the function as the biofertilizer. We found that, depending on the phosphate concentration in the medium, this bacterium produces copious amount of polyssacharide levan, a D-fructofuranosyl polymer, as an exopolysaccharide essential in the infection of the bacterium to the plants and in the protection of invasion of other bacteria in the environment.
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