| Project/Area Number |
24658077
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
KITA Keiko 京都大学, (連合)農学研究科(研究院), 教授 (70234226)
|
|---|
| Research Collaborator |
NAKAMURA Keigo 京都大学, 農学部, 4回生
HIOKI Takahiro 京都大学, 農学部, 4回生
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 制限酵素 / 分子工学 / DNA / ドメイン / リンカー / 分子間相互作用 / 構造機能相関 / 人工酵素 / ヌクレアーゼ |
|---|
| Research Abstract |
Restriction enzymes recognize and cleave DNA at specific site, and are both indispensable tools for gene manipulation and for studying sequence-specific interaction with DNA. Co-crystal structure of EcoT38I reveals several unique features for DNA recognition and catalysis. This study demonstrates that site-directed mutagenesis approach yielded a EcoT38I (GPuGCPyC) variant that preferentially cleaves one of the degenerate sequences (GAGCTC).
|
