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Analysis and application of lipid metabolisms in anaerobic bacteria for reducing trans fatty acid in foods derived from ruminant animals

Research Project

Project/Area Number 24658079
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

OGAWA Jun  京都大学, (連合)農学研究科(研究院), 教授 (70281102)

Co-Investigator(Kenkyū-buntansha) ANDO Akinori  京都大学, 生理化学研究ユニット, 助教 (10537765)
KISHINO Shigenobu  京都大学, 大学院農学研究科, 助教 (40432348)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsトランス脂肪酸 / 嫌気性微生物 / 飽和化 / 反芻動物 / FAD / NADH / オレイン酸 / 乳酸菌 / 嫌気性細菌 / レシチン / リン脂質 / 酵素反応
Research Abstract

Trans fatty acids in foods are reported to have bad effects on health. Trans fatty acids are found in foods derived from ruminant animals such as cow and sheep. Low trans fatty acid is recommended for health. In this research, it was found that hydratase catalyzing the first step of the polyunsaturated fatty acid saturation metabolism is involved in trans fatty acid generating systems. The ratio of cis/trans fatty acid was influenced by the activity control of the hydratase.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2013 2012 Other

All Journal Article (10 results) (of which Peer Reviewed: 3 results) Presentation (6 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Hydroxy fatty acid production by Pediococcus sp2013

    • Author(s)
      Takeuchi, M., S. Kishino, K. Tanabe, A. Hirata, S.B. Park, S. Shimizu, J. Ogawa
    • Journal Title

      Eur. J. Lipid Sci. Technol.

      Volume: 115(4) Issue: 4 Pages: 386-393

    • DOI

      10.1002/ejlt.201200414

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition.2013

    • Author(s)
      Kishino S, Takeuchi M, Park S, Hirata A, Kitamura N, Kunisawa J, Kiyono H, Iwamoto R, Isobe Y, Arita M, Arai H, Ueda K, Shima J, Takahashi S, Yokozeki K, Shimizu S, Ogawa J.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 110 Issue: 44 Pages: 17808-17813

    • DOI

      10.1073/pnas.1312937110

    • NAID

      120005341879

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 乳酸菌の新たな脂肪酸代謝―機能性脂肪酸生産と腸内脂質代謝制御への展開2013

    • Author(s)
      岸野重信,小川順
    • Journal Title

      化学と生物

      Volume: 51 (11) Pages: 738-744

    • Related Report
      2013 Final Research Report
  • [Journal Article] 乳酸菌における不飽和脂肪酸の飽和化反応に関わる新規酵素群の機能解析2013

    • Author(s)
      岸野重信,小川順
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 71 (2) Pages: 134-137

    • NAID

      10031159695

    • Related Report
      2013 Final Research Report
  • [Journal Article] 「特集 : 美味しい健康生活は微生物が作る~作物生産、食品素材開発、健康支援~」乳酸菌代謝と美味しい健康生活2013

    • Author(s)
      小川順,岸野重信
    • Journal Title

      生物工学会誌

      Volume: 91 (11) Pages: 637-640

    • Related Report
      2013 Final Research Report
  • [Journal Article] 腸内細菌に特異な脂質代謝「脂肪酸飽和化反応」に関与する新規酵素系の機能解析と応用2013

    • Author(s)
      小川順
    • Journal Title

      日本応用酵素協会誌

      Volume: 48 Pages: 47-48

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] 乳酸菌の新たな脂肪酸代謝―機能性脂肪酸生産と腸内脂質代謝制御への展開-2013

    • Author(s)
      岸野重信, 小川 順
    • Journal Title

      化学と生物

      Volume: 51 Pages: 738-744

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 乳酸菌における不飽和脂肪酸の飽和化反応に関わる新規酵素群の機能解析2013

    • Author(s)
      岸野重信, 小川 順
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 71 Pages: 134-137

    • NAID

      10031159695

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 乳酸菌代謝と美味しい健康生活2013

    • Author(s)
      小川 順, 岸野重信
    • Journal Title

      生物工学会誌

      Volume: 91 Pages: 637-640

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Polyunsaturated fatty acids production and transformation by Mortierella alpina and anaerobic bacteria2012

    • Author(s)
      Jun Ogawa
    • Journal Title

      European Journal of Lipid Science and Technology

      Volume: 114 Issue: 10 Pages: 1107-1113

    • DOI

      10.1002/ejlt.201200069

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Development of platform technologies and screening of module enzymes for multi-component enzyme systems requiring energy supply2013

    • Author(s)
      Ogawa, J., S. Kishino, M. Hibi, N. Horinouchi, K. Yokozeki, S. Takahashi, S. Shimizu
    • Organizer
      Enzyme Engineering XXII
    • Place of Presentation
      Toyama, Japan
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Enzymatic chiral alcohol synthesis using oxygenases and hydratases2013

    • Author(s)
      Ogawa, J., M. Hibi, S. Kishino, S. Shimizu
    • Organizer
      17^<th> German-Japanese Workshop on Enzyme Technology
    • Place of Presentation
      Hamburg, Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of multi-component enzyme systems for future chemical industry2013

    • Author(s)
      Ogawa, J., M. Hibi, S. Kishino, N. Horinouchi, A. Ando, E. Sakuradani, S. Shimizu
    • Organizer
      2013 KSBB Spring Meeting and International Symposium
    • Place of Presentation
      Gwangju, Korea
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Lactobacillus plantarum AKU 1009a由来リノール酸水和酵素の諸性質解明2013

    • Author(s)
      竹内道樹,田辺香緒里,平田晶子,朴時範,岸野重信,小川順
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] Enzymatic chiral alcohol synthesis using oxygenases and hydratases2013

    • Author(s)
      J. Ogawa, M. Hibi, S. Kishino, S. Shimizu
    • Organizer
      17th German-Japanese Workshop on Enzyme Technology
    • Place of Presentation
      Hamburg, Germany
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Lactobacillus plantarum AKU 1009a 由来リノール酸水和酵素の諸性質解明2013

    • Author(s)
      竹内 道樹
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.hakko.kais.kyoto-u.ac.jp/

    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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