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Molecular genetic analysis of the cyanobacterium that can utilize far-red light for photosynthesis using chlorophyll d

Research Project

Project/Area Number 24658080
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

TSUCHIYA Tohru  京都大学, 地球環境学堂, 准教授 (20362569)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords応用微生物 / シアノバクテリア / クロロフィルd / 光合成
Outline of Final Research Achievements

Acaryochloris marina is the cyanobacterium that can utilize far-red light for photosynthesis, whereas most other oxygenic photosynthetic organisms cannot use the light. However, the lack of molecular genetic techniques for A. marina prevented us from studying this organism deeply. In this project, we aimed to develop the molecular genetic techniques for A. marina in order to elucidate the adaptation mechanism of A. marina to far-red light utilization. In the result of this study, we succeeded in introducing a foreign gene into A. marina using an expression vector. We also succeeded in producing transposon-tagged mutants of A. marina by transposon tagging.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Acknowledgement Compliant: 2 results) Presentation (13 results) (of which Invited: 1 results)

  • [Journal Article] Artificially acquired chlorophyll b is highly acceptable to the thylakoid-lacking cyanobacterium, Gloeobacter violaceus PCC 7421.2014

    • Author(s)
      Araki, M., Akimoto, S., Mimuro, M., Tsuchiya, T.
    • Journal Title

      Plant Physiol. Biochem.

      Volume: 81 Pages: 155-162

    • DOI

      10.1016/j.plaphy.2014.01.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of a high-frequency in vivo transposon mutagenesis system for Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 79422014

    • Author(s)
      Watabe, K., Mimuro, M., Tsuchiya, T.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 1-2 Pages: 2017-2026

    • DOI

      10.1007/s11120-015-0082-4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Establishment of the reporter system for a thylakoid-lacking cyanobacterium, Gloeobacter violaceus PCC 74212013

    • Author(s)
      Araki, M., Shimada, Y., Mimuro, M., Tsuchiya, T.
    • Journal Title

      FEBS Open Bio

      Volume: 3 Issue: 1 Pages: 11-15

    • DOI

      10.1016/j.fob.2012.11.003

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Metabolic Engineering of the Chl d-Dominated Cyanobacterium Acaryochloris marina : Production of a Novel Chl Species by the Introduction of the Chlorophyllide a Oxygenase Gene2012

    • Author(s)
      Tohru Tsuchiya, Tadashi Mizoguchi, Seiji Akimoto, Tatsuya Tomo, Hitoshi Tamiaki, Mamoru Mimuro
    • Journal Title

      PLANT AND CELL PHYSIOLOGY

      Volume: 53 Issue: 3 Pages: 518-527

    • DOI

      10.1093/pcp/pcs007

    • NAID

      10030309263

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Artificial produced [7-formyl]-chlorophyll d functions as an antenna pigment in the photosystem II isolated from the chlorophyllide a oxygenase-expressing Acaryochloris marina2012

    • Author(s)
      T. Tsuchiya
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1817 Issue: 8 Pages: 1285-1291

    • DOI

      10.1016/j.bbabio.2012.02.021

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Opposite chilarity of α-carotene in unusual cyanobacteria with unique chlorophylls, Acaryochloris and Prochlorococcus2012

    • Author(s)
      Takaichi, S.
    • Journal Title

      Plant Cell Physiol.

      Volume: 53 Issue: 11 Pages: 1881-1888

    • DOI

      10.1093/pcp/pcs126

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 代謝工学による新たなクロロフィル分子の合成2012

    • Author(s)
      土屋 徹
    • Journal Title

      光合成研究

      Volume: 22 Pages: 106-114

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Molecular genetic analysis of the chlorophyll d-dominated cyanobacterium Acaryochloris marina2014

    • Author(s)
      Tsuchiya, T., Watabe, K.
    • Organizer
      International meeting "Photosynthesis Research for Sustainability - 2014"
    • Place of Presentation
      Pushchino (Russia)
    • Year and Date
      2014-06-02 – 2014-06-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 始原的シアノバクテリアGloeobacter violaceus PCC 7421は人工的に獲得させたクロロフィル bに寛容である2014

    • Author(s)
      荒木 美英、秋本 誠志、三室 守、土屋 徹
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] クロロフィルdを有するシアノバクテリアAcaryochloris marinaでの順遺伝学的解析のためのトランスポゾンタギング系の開発2014

    • Author(s)
      渡部 和幸、三室 守、土屋 徹
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Chlorophyll b biosynthesis in a genetically engineered Gloeobacter violaceus PCC 74212013

    • Author(s)
      Araki, M., Mimuro, M., Tsuchiya, T.
    • Organizer
      International meeting "Photosynthesis Research for Sustainability - 2013"
    • Place of Presentation
      Baku (Azerbaijan)
    • Related Report
      2013 Research-status Report
  • [Presentation] Development of a transformation system for a cyanobacterium, Gloeobacter violaceus PCC 7421 by conjugal gene transfer2013

    • Author(s)
      Araki, M., Mimuro, M., Tsuchiya, T.
    • Organizer
      International meeting "Photosynthesis Research for Sustainability - 2013"
    • Place of Presentation
      Baku (Azerbaijan)
    • Related Report
      2013 Research-status Report
  • [Presentation] Development of a highly-frequent in vivo transposon mutagenesis system for Synechocystis sp. PCC 68032013

    • Author(s)
      Watabe, K., Mimuro, M., Tsuchiya, T.
    • Organizer
      International meeting "Photosynthesis Research for Sustainability - 2013"
    • Place of Presentation
      Baku (Azerbaijan)
    • Related Report
      2013 Research-status Report
  • [Presentation] シアノバクテリアでの順遺伝学的解析を促進するツールの開発2013

    • Author(s)
      土屋 徹
    • Organizer
      ラン藻の分子生物学2013
    • Place of Presentation
      かずさアカデミアホール
    • Related Report
      2013 Research-status Report
  • [Presentation] Synechocystis sp. PCC 6803の順遺伝学的解析のための高効率なin vivoトランスポゾンタギング系の開発2013

    • Author(s)
      渡部 和幸、三室 守、土屋 徹
    • Organizer
      ラン藻の分子生物学2013
    • Place of Presentation
      かずさアカデミアホール
    • Related Report
      2013 Research-status Report
  • [Presentation] 代謝工学的アプローチから探る光化学系IIの色素組成の柔軟性2013

    • Author(s)
      土屋 徹
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Gloeobacter violaceus PCC 7421でのトランスポゾンタギング系の開発2013

    • Author(s)
      荒木 美英、三室 守、土屋 徹
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Synechocystis sp. PCC 6803における順遺伝学的解析のためのトランスポゾンタギング系の高効率化2012

    • Author(s)
      渡部 和幸、三室 守、土屋 徹
    • Organizer
      第20回光合成の色素系と反応中心に関するセミナー
    • Place of Presentation
      大阪大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Alteration of the chlorophyll composition of the chlorophyll d-dominated cyanobacterium Acaryochloris marina2012

    • Author(s)
      Tsuchiya, T.
    • Organizer
      Second International Symposium on Biosynthesis of Tetrapyrroles
    • Place of Presentation
      立命館大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 新奇なシアノバクテリアでの形質転換系の開発とその応用

    • Author(s)
      土屋 徹
    • Organizer
      藻類談話会
    • Place of Presentation
      神戸大学
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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